Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20110 and BPHYT_RS20115 are separated by 25 nucleotides BPHYT_RS20115 and BPHYT_RS20120 are separated by 182 nucleotides BPHYT_RS20120 and BPHYT_RS20125 are separated by 396 nucleotides
BPHYT_RS20110: BPHYT_RS20110 - sensor histidine kinase, at 54,486 to 55,868
_RS20110
BPHYT_RS20115: BPHYT_RS20115 - transcriptional regulator, at 55,894 to 56,592
_RS20115
BPHYT_RS20120: BPHYT_RS20120 - porin, at 56,775 to 57,899
_RS20120
BPHYT_RS20125: BPHYT_RS20125 - histidine kinase, at 58,296 to 58,511
_RS20125
Position (kb) 56
57
58 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 55.877 kb on + strand at 56.026 kb on + strand, within BPHYT_RS20115 at 56.469 kb on + strand, within BPHYT_RS20115 at 56.475 kb on + strand, within BPHYT_RS20115 at 56.625 kb on - strand at 56.930 kb on + strand, within BPHYT_RS20120 at 57.318 kb on + strand, within BPHYT_RS20120 at 57.528 kb on + strand, within BPHYT_RS20120 at 57.808 kb on - strand at 57.974 kb on + strand at 57.975 kb on - strand at 58.135 kb on - strand at 58.696 kb on - strand at 58.897 kb on + strand at 58.898 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 55,877 + -0.3 56,026 + BPHYT_RS20115 0.19 +3.4 56,469 + BPHYT_RS20115 0.82 -0.3 56,475 + BPHYT_RS20115 0.83 +0.0 56,625 - -1.4 56,930 + BPHYT_RS20120 0.14 -0.1 57,318 + BPHYT_RS20120 0.48 -1.0 57,528 + BPHYT_RS20120 0.67 -2.0 57,808 - +0.6 57,974 + +0.2 57,975 - +0.7 58,135 - +1.4 58,696 - +1.1 58,897 + -1.3 58,898 - +2.0
Or see this region's nucleotide sequence