Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19825 and BPHYT_RS19830 are separated by 174 nucleotides BPHYT_RS19830 and BPHYT_RS19835 are separated by 370 nucleotides BPHYT_RS19835 and BPHYT_RS19840 are separated by 138 nucleotides
BPHYT_RS19825: BPHYT_RS19825 - integrase, at 4,461,074 to 4,462,288
_RS19825
BPHYT_RS19830: BPHYT_RS19830 - tRNA modification GTPase TrmE, at 4,462,463 to 4,463,857
_RS19830
BPHYT_RS19835: BPHYT_RS19835 - XRE family transcriptional regulator, at 4,464,228 to 4,464,635
_RS19835
BPHYT_RS19840: BPHYT_RS19840 - membrane protein insertase YidC, at 4,464,774 to 4,466,432
_RS19840
Position (kb)
4462
4463
4464 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4461.747 kb on + strand, within BPHYT_RS19825 at 4461.748 kb on - strand, within BPHYT_RS19825 at 4461.779 kb on - strand, within BPHYT_RS19825 at 4461.825 kb on - strand, within BPHYT_RS19825 at 4462.060 kb on - strand, within BPHYT_RS19825 at 4462.150 kb on - strand, within BPHYT_RS19825 at 4462.221 kb on + strand at 4462.331 kb on - strand at 4462.419 kb on + strand at 4464.024 kb on - strand at 4464.041 kb on + strand at 4464.042 kb on - strand at 4464.046 kb on - strand at 4464.056 kb on - strand at 4464.151 kb on - strand at 4464.336 kb on + strand, within BPHYT_RS19835 at 4464.374 kb on + strand, within BPHYT_RS19835 at 4464.374 kb on + strand, within BPHYT_RS19835 at 4464.375 kb on - strand, within BPHYT_RS19835 at 4464.375 kb on - strand, within BPHYT_RS19835 at 4464.419 kb on + strand, within BPHYT_RS19835 at 4464.463 kb on - strand, within BPHYT_RS19835 at 4464.513 kb on + strand, within BPHYT_RS19835 at 4464.610 kb on - strand at 4464.633 kb on + strand at 4464.633 kb on + strand at 4464.713 kb on - strand at 4464.722 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 4,461,747 + BPHYT_RS19825 0.55 +1.8 4,461,748 - BPHYT_RS19825 0.55 +0.0 4,461,779 - BPHYT_RS19825 0.58 +1.8 4,461,825 - BPHYT_RS19825 0.62 -1.4 4,462,060 - BPHYT_RS19825 0.81 -0.5 4,462,150 - BPHYT_RS19825 0.89 -0.8 4,462,221 + -0.5 4,462,331 - -1.7 4,462,419 + +0.1 4,464,024 - -0.7 4,464,041 + +0.4 4,464,042 - +0.1 4,464,046 - -0.8 4,464,056 - +0.3 4,464,151 - -0.2 4,464,336 + BPHYT_RS19835 0.26 +0.8 4,464,374 + BPHYT_RS19835 0.36 +0.4 4,464,374 + BPHYT_RS19835 0.36 +0.5 4,464,375 - BPHYT_RS19835 0.36 +0.1 4,464,375 - BPHYT_RS19835 0.36 -2.7 4,464,419 + BPHYT_RS19835 0.47 +0.8 4,464,463 - BPHYT_RS19835 0.58 -2.5 4,464,513 + BPHYT_RS19835 0.70 -0.0 4,464,610 - -0.4 4,464,633 + +1.4 4,464,633 + -0.1 4,464,713 - +0.4 4,464,722 - -0.3
Or see this region's nucleotide sequence