Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16715 and BPHYT_RS16720 are separated by 89 nucleotides BPHYT_RS16720 and BPHYT_RS16725 are separated by 10 nucleotides BPHYT_RS16725 and BPHYT_RS16730 are separated by 259 nucleotides BPHYT_RS16730 and BPHYT_RS16735 are separated by 25 nucleotides
BPHYT_RS16715: BPHYT_RS16715 - adenylosuccinate lyase, at 3,784,070 to 3,785,458
_RS16715
BPHYT_RS16720: BPHYT_RS16720 - Gluconokinase (EC 2.7.1.12) (from data) , at 3,785,548 to 3,786,045
_RS16720
BPHYT_RS16725: BPHYT_RS16725 - gluconate:H+ symporter (gntT) (from data) , at 3,786,056 to 3,787,453
_RS16725
BPHYT_RS16730: BPHYT_RS16730 - 2-dehydro-3-deoxyphosphogluconate aldolase, at 3,787,713 to 3,788,354
_RS16730
BPHYT_RS16735: BPHYT_RS16735 - phosphogluconate dehydratase, at 3,788,380 to 3,790,284
_RS16735
Position (kb)
3786
3787
3788 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3785.456 kb on + strand at 3785.653 kb on - strand, within BPHYT_RS16720 at 3785.745 kb on - strand, within BPHYT_RS16720 at 3785.745 kb on - strand, within BPHYT_RS16720 at 3786.058 kb on - strand at 3786.058 kb on - strand at 3786.162 kb on + strand at 3786.162 kb on + strand at 3786.163 kb on - strand at 3786.163 kb on - strand at 3786.163 kb on - strand at 3786.733 kb on + strand, within BPHYT_RS16725 at 3786.734 kb on - strand, within BPHYT_RS16725 at 3786.734 kb on - strand, within BPHYT_RS16725 at 3786.734 kb on - strand, within BPHYT_RS16725 at 3786.741 kb on - strand, within BPHYT_RS16725 at 3786.741 kb on - strand, within BPHYT_RS16725 at 3786.741 kb on - strand, within BPHYT_RS16725 at 3786.834 kb on + strand, within BPHYT_RS16725 at 3786.835 kb on - strand, within BPHYT_RS16725 at 3786.835 kb on - strand, within BPHYT_RS16725 at 3786.904 kb on - strand, within BPHYT_RS16725 at 3787.264 kb on + strand, within BPHYT_RS16725 at 3787.365 kb on + strand at 3787.365 kb on + strand at 3787.374 kb on + strand at 3787.374 kb on + strand at 3787.375 kb on - strand at 3787.375 kb on - strand at 3787.375 kb on - strand at 3787.375 kb on - strand at 3787.375 kb on - strand at 3787.375 kb on - strand at 3787.375 kb on - strand at 3787.581 kb on + strand at 3787.582 kb on - strand at 3787.652 kb on - strand at 3787.714 kb on + strand at 3787.715 kb on - strand at 3787.715 kb on - strand at 3788.355 kb on - strand at 3788.355 kb on - strand at 3788.382 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 3,785,456 + -1.4 3,785,653 - BPHYT_RS16720 0.21 -0.1 3,785,745 - BPHYT_RS16720 0.40 -0.0 3,785,745 - BPHYT_RS16720 0.40 +0.2 3,786,058 - +0.2 3,786,058 - +0.7 3,786,162 + +0.4 3,786,162 + -0.1 3,786,163 - +0.1 3,786,163 - -0.7 3,786,163 - -0.9 3,786,733 + BPHYT_RS16725 0.48 -0.5 3,786,734 - BPHYT_RS16725 0.48 -1.1 3,786,734 - BPHYT_RS16725 0.48 -0.4 3,786,734 - BPHYT_RS16725 0.48 +0.2 3,786,741 - BPHYT_RS16725 0.49 -0.4 3,786,741 - BPHYT_RS16725 0.49 -0.3 3,786,741 - BPHYT_RS16725 0.49 -1.2 3,786,834 + BPHYT_RS16725 0.56 +1.7 3,786,835 - BPHYT_RS16725 0.56 -0.2 3,786,835 - BPHYT_RS16725 0.56 -1.0 3,786,904 - BPHYT_RS16725 0.61 -0.3 3,787,264 + BPHYT_RS16725 0.86 -1.1 3,787,365 + -0.3 3,787,365 + +0.2 3,787,374 + -0.1 3,787,374 + -1.0 3,787,375 - +0.3 3,787,375 - +0.7 3,787,375 - -0.7 3,787,375 - -1.0 3,787,375 - -0.4 3,787,375 - +0.0 3,787,375 - -0.5 3,787,581 + -0.1 3,787,582 - -0.4 3,787,652 - -0.1 3,787,714 + +0.8 3,787,715 - -1.2 3,787,715 - -1.4 3,788,355 - -0.5 3,788,355 - +3.9 3,788,382 - +0.1
Or see this region's nucleotide sequence