Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13475 and BPHYT_RS13480 are separated by 170 nucleotides BPHYT_RS13480 and BPHYT_RS13485 are separated by 112 nucleotides BPHYT_RS13485 and BPHYT_RS13490 are separated by 360 nucleotides BPHYT_RS13490 and BPHYT_RS13495 are separated by 30 nucleotides
BPHYT_RS13475: BPHYT_RS13475 - 2-keto-gluconate dehydrogenase, at 3,075,425 to 3,077,011
_RS13475
BPHYT_RS13480: BPHYT_RS13480 - hypothetical protein, at 3,077,182 to 3,077,853
_RS13480
BPHYT_RS13485: BPHYT_RS13485 - lactoylglutathione lyase, at 3,077,966 to 3,078,364
_RS13485
BPHYT_RS13490: BPHYT_RS13490 - hypothetical protein, at 3,078,725 to 3,079,135
_RS13490
BPHYT_RS13495: BPHYT_RS13495 - AsnC family transcriptional regulator, at 3,079,166 to 3,079,690
_RS13495
Position (kb)
3077
3078
3079 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3077.094 kb on + strand at 3077.136 kb on - strand at 3077.183 kb on + strand at 3077.929 kb on - strand at 3077.937 kb on + strand at 3077.964 kb on + strand at 3077.964 kb on + strand at 3078.241 kb on - strand at 3078.654 kb on - strand at 3078.676 kb on + strand at 3078.908 kb on + strand, within BPHYT_RS13490 at 3078.909 kb on - strand, within BPHYT_RS13490 at 3078.981 kb on - strand, within BPHYT_RS13490 at 3079.201 kb on + strand at 3079.201 kb on + strand at 3079.202 kb on - strand at 3079.202 kb on - strand at 3079.215 kb on + strand at 3079.216 kb on - strand at 3079.216 kb on - strand at 3079.353 kb on - strand, within BPHYT_RS13495
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 3,077,094 + -0.8 3,077,136 - +1.8 3,077,183 + -2.2 3,077,929 - +0.2 3,077,937 + +1.8 3,077,964 + -0.6 3,077,964 + +1.0 3,078,241 - -1.0 3,078,654 - -0.1 3,078,676 + +1.2 3,078,908 + BPHYT_RS13490 0.45 -0.4 3,078,909 - BPHYT_RS13490 0.45 -0.5 3,078,981 - BPHYT_RS13490 0.62 -0.3 3,079,201 + +2.0 3,079,201 + +0.4 3,079,202 - -0.1 3,079,202 - -1.3 3,079,215 + +0.2 3,079,216 - +0.8 3,079,216 - -0.1 3,079,353 - BPHYT_RS13495 0.36 +2.4
Or see this region's nucleotide sequence