Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10690 and BPHYT_RS10695 overlap by 4 nucleotides BPHYT_RS10695 and BPHYT_RS10700 are separated by 32 nucleotides BPHYT_RS10700 and BPHYT_RS10705 are separated by 40 nucleotides BPHYT_RS10705 and BPHYT_RS10710 are separated by 10 nucleotides
BPHYT_RS10690: BPHYT_RS10690 - Rieske (2Fe-2S) protein, at 2,433,692 to 2,434,975
_RS10690
BPHYT_RS10695: BPHYT_RS10695 - aromatic-ring-hydroxylating dioxygenase subunit beta, at 2,434,972 to 2,435,472
_RS10695
BPHYT_RS10700: BPHYT_RS10700 - 4-oxalocrotonate tautomerase, at 2,435,505 to 2,435,888
_RS10700
BPHYT_RS10705: BPHYT_RS10705 - MFS transporter, at 2,435,929 to 2,437,245
_RS10705
BPHYT_RS10710: BPHYT_RS10710 - sensor histidine kinase, at 2,437,256 to 2,438,776
_RS10710
Position (kb)
2435
2436
2437
2438 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2435.108 kb on - strand, within BPHYT_RS10695 at 2435.510 kb on + strand at 2435.511 kb on - strand at 2436.430 kb on + strand, within BPHYT_RS10705 at 2436.877 kb on + strand, within BPHYT_RS10705 at 2436.878 kb on - strand, within BPHYT_RS10705 at 2436.879 kb on + strand, within BPHYT_RS10705 at 2437.132 kb on + strand at 2437.132 kb on + strand at 2437.227 kb on + strand at 2437.645 kb on - strand, within BPHYT_RS10710 at 2437.980 kb on + strand, within BPHYT_RS10710
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 2,435,108 - BPHYT_RS10695 0.27 -0.4 2,435,510 + +0.5 2,435,511 - -0.9 2,436,430 + BPHYT_RS10705 0.38 -0.6 2,436,877 + BPHYT_RS10705 0.72 -1.3 2,436,878 - BPHYT_RS10705 0.72 +0.4 2,436,879 + BPHYT_RS10705 0.72 +1.8 2,437,132 + +0.1 2,437,132 + -0.5 2,437,227 + -0.0 2,437,645 - BPHYT_RS10710 0.26 -2.1 2,437,980 + BPHYT_RS10710 0.48 -1.2
Or see this region's nucleotide sequence