Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09860 and BPHYT_RS09865 are separated by 60 nucleotides BPHYT_RS09865 and BPHYT_RS09870 are separated by 143 nucleotides BPHYT_RS09870 and BPHYT_RS09875 are separated by 285 nucleotides
BPHYT_RS09860: BPHYT_RS09860 - pilus assembly protein PilP, at 2,243,674 to 2,244,348
_RS09860
BPHYT_RS09865: BPHYT_RS09865 - methyltransferase, at 2,244,409 to 2,244,897
_RS09865
BPHYT_RS09870: BPHYT_RS09870 - membrane protein, at 2,245,041 to 2,246,198
_RS09870
BPHYT_RS09875: BPHYT_RS09875 - aldehyde dehydrogenase, at 2,246,484 to 2,247,929
_RS09875
Position (kb)
2245
2246
2247 Strain fitness (log2 ratio)
-1
0
1
2 at 2244.110 kb on - strand, within BPHYT_RS09860 at 2245.115 kb on + strand at 2245.115 kb on + strand at 2245.166 kb on - strand, within BPHYT_RS09870 at 2245.283 kb on - strand, within BPHYT_RS09870 at 2245.503 kb on - strand, within BPHYT_RS09870 at 2245.577 kb on + strand, within BPHYT_RS09870 at 2245.579 kb on + strand, within BPHYT_RS09870 at 2245.823 kb on - strand, within BPHYT_RS09870 at 2245.841 kb on - strand, within BPHYT_RS09870 at 2246.219 kb on - strand at 2246.947 kb on - strand, within BPHYT_RS09875 at 2247.128 kb on - strand, within BPHYT_RS09875
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 2,244,110 - BPHYT_RS09860 0.65 +1.6 2,245,115 + +1.9 2,245,115 + -1.1 2,245,166 - BPHYT_RS09870 0.11 +2.7 2,245,283 - BPHYT_RS09870 0.21 -1.5 2,245,503 - BPHYT_RS09870 0.40 +0.8 2,245,577 + BPHYT_RS09870 0.46 -1.1 2,245,579 + BPHYT_RS09870 0.46 -1.7 2,245,823 - BPHYT_RS09870 0.68 -1.0 2,245,841 - BPHYT_RS09870 0.69 -0.8 2,246,219 - +0.4 2,246,947 - BPHYT_RS09875 0.32 -0.5 2,247,128 - BPHYT_RS09875 0.45 +0.4
Or see this region's nucleotide sequence