Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07125 and BPHYT_RS07130 are separated by 12 nucleotides BPHYT_RS07130 and BPHYT_RS07135 are separated by 24 nucleotides BPHYT_RS07135 and BPHYT_RS07140 are separated by 9 nucleotides BPHYT_RS07140 and BPHYT_RS07145 are separated by 297 nucleotides
BPHYT_RS07125: BPHYT_RS07125 - biopolymer transporter ExbB, at 1,616,357 to 1,617,262
_RS07125
BPHYT_RS07130: BPHYT_RS07130 - biopolymer transporter ExbD, at 1,617,275 to 1,617,697
_RS07130
BPHYT_RS07135: BPHYT_RS07135 - biopolymer transporter ExbD, at 1,617,722 to 1,618,150
_RS07135
BPHYT_RS07140: BPHYT_RS07140 - hypothetical protein, at 1,618,160 to 1,619,344
_RS07140
BPHYT_RS07145: BPHYT_RS07145 - DNA-binding protein, at 1,619,642 to 1,620,217
_RS07145
Position (kb) 1618
1619
1620 Strain fitness (log2 ratio) -1
0
1 at 1617.460 kb on + strand, within BPHYT_RS07130 at 1618.305 kb on - strand, within BPHYT_RS07140 at 1618.619 kb on + strand, within BPHYT_RS07140 at 1618.923 kb on - strand, within BPHYT_RS07140 at 1619.238 kb on - strand at 1619.684 kb on - strand at 1620.105 kb on + strand, within BPHYT_RS07145
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 1,617,460 + BPHYT_RS07130 0.44 +0.8 1,618,305 - BPHYT_RS07140 0.12 +0.7 1,618,619 + BPHYT_RS07140 0.39 +0.7 1,618,923 - BPHYT_RS07140 0.64 +1.2 1,619,238 - +0.8 1,619,684 - -0.9 1,620,105 + BPHYT_RS07145 0.80 +0.1
Or see this region's nucleotide sequence