Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00630 and BPHYT_RS00635 overlap by 4 nucleotides BPHYT_RS00635 and BPHYT_RS00640 are separated by 55 nucleotides BPHYT_RS00640 and BPHYT_RS00645 are separated by 25 nucleotides
BPHYT_RS00630: BPHYT_RS00630 - 3-oxoacyl-ACP synthase, at 149,904 to 150,965
_RS00630
BPHYT_RS00635: BPHYT_RS00635 - acetyltransferase, at 150,962 to 151,540
_RS00635
BPHYT_RS00640: BPHYT_RS00640 - acyl-CoA dehydrogenase, at 151,596 to 152,684
_RS00640
BPHYT_RS00645: BPHYT_RS00645 - diaminopimelate decarboxylase, at 152,710 to 153,918
_RS00645
Position (kb) 151
152
153 Strain fitness (log2 ratio) -2
-1
0
1
2 at 150.617 kb on + strand, within BPHYT_RS00630 at 150.617 kb on + strand, within BPHYT_RS00630 at 150.617 kb on + strand, within BPHYT_RS00630 at 150.617 kb on + strand, within BPHYT_RS00630 at 150.617 kb on + strand, within BPHYT_RS00630 at 150.618 kb on - strand, within BPHYT_RS00630 at 150.823 kb on + strand, within BPHYT_RS00630 at 150.920 kb on + strand at 150.921 kb on - strand at 151.020 kb on + strand, within BPHYT_RS00635 at 151.021 kb on - strand, within BPHYT_RS00635 at 151.021 kb on - strand, within BPHYT_RS00635 at 151.118 kb on + strand, within BPHYT_RS00635 at 151.119 kb on - strand, within BPHYT_RS00635 at 151.119 kb on - strand at 151.591 kb on - strand at 151.591 kb on - strand at 151.591 kb on - strand at 151.591 kb on - strand at 151.702 kb on + strand at 151.702 kb on + strand at 151.703 kb on - strand at 151.783 kb on - strand, within BPHYT_RS00640 at 151.833 kb on + strand, within BPHYT_RS00640 at 151.833 kb on + strand at 151.833 kb on + strand, within BPHYT_RS00640 at 151.834 kb on - strand, within BPHYT_RS00640 at 151.933 kb on + strand, within BPHYT_RS00640 at 151.990 kb on + strand, within BPHYT_RS00640 at 151.990 kb on + strand, within BPHYT_RS00640 at 151.991 kb on - strand, within BPHYT_RS00640 at 152.070 kb on - strand, within BPHYT_RS00640 at 152.099 kb on - strand, within BPHYT_RS00640 at 152.099 kb on - strand, within BPHYT_RS00640 at 152.144 kb on - strand, within BPHYT_RS00640 at 152.166 kb on + strand, within BPHYT_RS00640 at 152.309 kb on - strand, within BPHYT_RS00640 at 152.309 kb on - strand, within BPHYT_RS00640 at 152.318 kb on + strand, within BPHYT_RS00640 at 152.319 kb on - strand, within BPHYT_RS00640 at 152.351 kb on - strand, within BPHYT_RS00640 at 152.650 kb on - strand at 152.650 kb on - strand at 152.880 kb on - strand, within BPHYT_RS00645 at 152.897 kb on - strand, within BPHYT_RS00645 at 152.897 kb on - strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.051 kb on - strand, within BPHYT_RS00645 at 153.051 kb on - strand, within BPHYT_RS00645 at 153.068 kb on - strand, within BPHYT_RS00645 at 153.074 kb on + strand, within BPHYT_RS00645 at 153.075 kb on - strand, within BPHYT_RS00645 at 153.229 kb on + strand, within BPHYT_RS00645 at 153.229 kb on + strand, within BPHYT_RS00645 at 153.230 kb on - strand, within BPHYT_RS00645
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 150,617 + BPHYT_RS00630 0.67 -0.4 150,617 + BPHYT_RS00630 0.67 +0.4 150,617 + BPHYT_RS00630 0.67 -0.2 150,617 + BPHYT_RS00630 0.67 +1.2 150,617 + BPHYT_RS00630 0.67 -0.0 150,618 - BPHYT_RS00630 0.67 -0.8 150,823 + BPHYT_RS00630 0.87 -0.1 150,920 + +0.0 150,921 - +0.6 151,020 + BPHYT_RS00635 0.10 +0.7 151,021 - BPHYT_RS00635 0.10 -0.8 151,021 - BPHYT_RS00635 0.10 +0.9 151,118 + BPHYT_RS00635 0.27 -0.4 151,119 - BPHYT_RS00635 0.27 -0.1 151,119 - +2.2 151,591 - +0.9 151,591 - -0.1 151,591 - -0.2 151,591 - +0.0 151,702 + -0.8 151,702 + +0.1 151,703 - +0.1 151,783 - BPHYT_RS00640 0.17 +0.9 151,833 + BPHYT_RS00640 0.22 -1.6 151,833 + +0.1 151,833 + BPHYT_RS00640 0.22 +0.3 151,834 - BPHYT_RS00640 0.22 +0.5 151,933 + BPHYT_RS00640 0.31 -0.1 151,990 + BPHYT_RS00640 0.36 -0.4 151,990 + BPHYT_RS00640 0.36 +0.0 151,991 - BPHYT_RS00640 0.36 -0.1 152,070 - BPHYT_RS00640 0.44 +0.5 152,099 - BPHYT_RS00640 0.46 -1.2 152,099 - BPHYT_RS00640 0.46 -1.9 152,144 - BPHYT_RS00640 0.50 -1.1 152,166 + BPHYT_RS00640 0.52 -1.4 152,309 - BPHYT_RS00640 0.65 +0.4 152,309 - BPHYT_RS00640 0.65 -0.5 152,318 + BPHYT_RS00640 0.66 -0.1 152,319 - BPHYT_RS00640 0.66 +0.3 152,351 - BPHYT_RS00640 0.69 -1.5 152,650 - -0.1 152,650 - +0.4 152,880 - BPHYT_RS00645 0.14 -0.1 152,897 - BPHYT_RS00645 0.15 -0.6 152,897 - BPHYT_RS00645 0.15 -0.6 153,050 + BPHYT_RS00645 0.28 -0.9 153,050 + BPHYT_RS00645 0.28 -1.1 153,050 + BPHYT_RS00645 0.28 -0.4 153,050 + BPHYT_RS00645 0.28 -1.0 153,050 + BPHYT_RS00645 0.28 -0.3 153,050 + BPHYT_RS00645 0.28 -1.5 153,050 + BPHYT_RS00645 0.28 -1.0 153,051 - BPHYT_RS00645 0.28 -0.1 153,051 - BPHYT_RS00645 0.28 -1.3 153,068 - BPHYT_RS00645 0.30 +0.2 153,074 + BPHYT_RS00645 0.30 -0.3 153,075 - BPHYT_RS00645 0.30 -0.4 153,229 + BPHYT_RS00645 0.43 +0.2 153,229 + BPHYT_RS00645 0.43 -0.7 153,230 - BPHYT_RS00645 0.43 -0.7
Or see this region's nucleotide sequence