Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00335 and BPHYT_RS00340 are separated by 16 nucleotides BPHYT_RS00340 and BPHYT_RS00345 are separated by 223 nucleotides BPHYT_RS00345 and BPHYT_RS00350 are separated by 312 nucleotides
BPHYT_RS00335: BPHYT_RS00335 - peptidase M19, at 84,101 to 85,099
_RS00335
BPHYT_RS00340: BPHYT_RS00340 - hypothetical protein, at 85,116 to 85,709
_RS00340
BPHYT_RS00345: BPHYT_RS00345 - MFS transporter, at 85,933 to 87,156
_RS00345
BPHYT_RS00350: BPHYT_RS00350 - hypothetical protein, at 87,469 to 88,155
_RS00350
Position (kb) 85
86
87
88 Strain fitness (log2 ratio) -2
-1
0
1
2 at 84.956 kb on - strand, within BPHYT_RS00335 at 84.986 kb on - strand, within BPHYT_RS00335 at 85.134 kb on - strand at 85.134 kb on - strand at 85.230 kb on - strand, within BPHYT_RS00340 at 85.466 kb on - strand, within BPHYT_RS00340 at 85.603 kb on - strand, within BPHYT_RS00340 at 85.777 kb on + strand at 85.777 kb on + strand at 85.778 kb on - strand at 85.786 kb on - strand at 85.927 kb on - strand at 85.934 kb on + strand at 85.951 kb on - strand at 85.951 kb on - strand at 86.086 kb on + strand, within BPHYT_RS00345 at 86.087 kb on - strand, within BPHYT_RS00345 at 86.110 kb on - strand, within BPHYT_RS00345 at 86.238 kb on + strand, within BPHYT_RS00345 at 86.239 kb on - strand, within BPHYT_RS00345 at 86.246 kb on + strand, within BPHYT_RS00345 at 86.259 kb on + strand, within BPHYT_RS00345 at 86.260 kb on - strand, within BPHYT_RS00345 at 86.332 kb on + strand, within BPHYT_RS00345 at 86.383 kb on + strand, within BPHYT_RS00345 at 86.443 kb on + strand, within BPHYT_RS00345 at 86.527 kb on + strand, within BPHYT_RS00345 at 86.527 kb on + strand, within BPHYT_RS00345 at 86.672 kb on - strand, within BPHYT_RS00345 at 86.672 kb on - strand, within BPHYT_RS00345 at 86.698 kb on + strand, within BPHYT_RS00345 at 86.698 kb on + strand, within BPHYT_RS00345 at 86.728 kb on + strand, within BPHYT_RS00345 at 86.729 kb on - strand, within BPHYT_RS00345 at 86.912 kb on + strand, within BPHYT_RS00345 at 86.913 kb on - strand, within BPHYT_RS00345 at 86.916 kb on - strand, within BPHYT_RS00345 at 86.916 kb on - strand, within BPHYT_RS00345 at 86.941 kb on + strand, within BPHYT_RS00345 at 86.942 kb on - strand, within BPHYT_RS00345 at 87.022 kb on - strand, within BPHYT_RS00345 at 87.025 kb on + strand, within BPHYT_RS00345 at 87.032 kb on + strand, within BPHYT_RS00345 at 87.033 kb on - strand, within BPHYT_RS00345 at 87.118 kb on + strand at 87.119 kb on - strand at 87.210 kb on - strand at 87.484 kb on + strand at 87.485 kb on - strand at 87.549 kb on + strand, within BPHYT_RS00350 at 87.738 kb on - strand, within BPHYT_RS00350 at 87.910 kb on - strand, within BPHYT_RS00350 at 88.010 kb on + strand, within BPHYT_RS00350
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 84,956 - BPHYT_RS00335 0.86 -1.6 84,986 - BPHYT_RS00335 0.89 +2.4 85,134 - -1.1 85,134 - -1.0 85,230 - BPHYT_RS00340 0.19 -0.4 85,466 - BPHYT_RS00340 0.59 +0.3 85,603 - BPHYT_RS00340 0.82 -1.4 85,777 + +0.4 85,777 + +0.4 85,778 - +0.5 85,786 - -1.8 85,927 - -1.0 85,934 + -0.3 85,951 - -0.4 85,951 - -1.3 86,086 + BPHYT_RS00345 0.12 +0.4 86,087 - BPHYT_RS00345 0.13 -0.9 86,110 - BPHYT_RS00345 0.14 -0.6 86,238 + BPHYT_RS00345 0.25 -1.7 86,239 - BPHYT_RS00345 0.25 -0.7 86,246 + BPHYT_RS00345 0.26 -0.6 86,259 + BPHYT_RS00345 0.27 +0.6 86,260 - BPHYT_RS00345 0.27 -0.8 86,332 + BPHYT_RS00345 0.33 -0.7 86,383 + BPHYT_RS00345 0.37 -1.0 86,443 + BPHYT_RS00345 0.42 +0.1 86,527 + BPHYT_RS00345 0.49 -0.9 86,527 + BPHYT_RS00345 0.49 -0.3 86,672 - BPHYT_RS00345 0.60 +0.7 86,672 - BPHYT_RS00345 0.60 +0.4 86,698 + BPHYT_RS00345 0.62 -0.6 86,698 + BPHYT_RS00345 0.62 -0.4 86,728 + BPHYT_RS00345 0.65 -0.2 86,729 - BPHYT_RS00345 0.65 +0.0 86,912 + BPHYT_RS00345 0.80 -0.4 86,913 - BPHYT_RS00345 0.80 +1.7 86,916 - BPHYT_RS00345 0.80 -0.2 86,916 - BPHYT_RS00345 0.80 -0.8 86,941 + BPHYT_RS00345 0.82 -0.6 86,942 - BPHYT_RS00345 0.82 -1.7 87,022 - BPHYT_RS00345 0.89 +0.1 87,025 + BPHYT_RS00345 0.89 +1.4 87,032 + BPHYT_RS00345 0.90 -2.1 87,033 - BPHYT_RS00345 0.90 -0.4 87,118 + -1.0 87,119 - -0.1 87,210 - +0.2 87,484 + +0.4 87,485 - -1.6 87,549 + BPHYT_RS00350 0.12 -0.3 87,738 - BPHYT_RS00350 0.39 +0.9 87,910 - BPHYT_RS00350 0.64 +1.8 88,010 + BPHYT_RS00350 0.79 +0.7
Or see this region's nucleotide sequence