Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32880

Experiment: 36hr PHYTO8 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32875 and BPHYT_RS32880 are separated by 22 nucleotidesBPHYT_RS32880 and BPHYT_RS32885 are separated by 651 nucleotides BPHYT_RS32875: BPHYT_RS32875 - GCN5 family acetyltransferase, at 2,954,950 to 2,955,762 _RS32875 BPHYT_RS32880: BPHYT_RS32880 - alanine acetyltransferase, at 2,955,785 to 2,956,360 _RS32880 BPHYT_RS32885: BPHYT_RS32885 - methyl-accepting chemotaxis protein, at 2,957,012 to 2,958,763 _RS32885 Position (kb) 2955 2956 2957Strain fitness (log2 ratio) -2 -1 0 1at 2955.416 kb on - strand, within BPHYT_RS32875at 2955.419 kb on + strand, within BPHYT_RS32875at 2955.806 kb on - strandat 2956.257 kb on - strand, within BPHYT_RS32880at 2956.283 kb on - strand, within BPHYT_RS32880at 2956.361 kb on - strandat 2956.894 kb on + strandat 2956.895 kb on - strandat 2957.083 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 0.1
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2,955,416 - BPHYT_RS32875 0.57 -2.1
2,955,419 + BPHYT_RS32875 0.58 +0.3
2,955,806 - -1.7
2,956,257 - BPHYT_RS32880 0.82 -1.1
2,956,283 - BPHYT_RS32880 0.86 -1.7
2,956,361 - -1.0
2,956,894 + +0.2
2,956,895 - -0.3
2,957,083 + -0.4

Or see this region's nucleotide sequence