Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32435

Experiment: 36hr PHYTO8 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32425 and BPHYT_RS32430 are separated by 111 nucleotidesBPHYT_RS32430 and BPHYT_RS32435 overlap by 14 nucleotidesBPHYT_RS32435 and BPHYT_RS32440 are separated by 9 nucleotides BPHYT_RS32425: BPHYT_RS32425 - formate dehydrogenase accessory protein FdhE, at 2,847,856 to 2,848,851 _RS32425 BPHYT_RS32430: BPHYT_RS32430 - formate dehydrogenase-N subunit gamma, at 2,848,963 to 2,849,619 _RS32430 BPHYT_RS32435: BPHYT_RS32435 - formate dehydrogenase-N subunit beta, at 2,849,606 to 2,850,538 _RS32435 BPHYT_RS32440: BPHYT_RS32440 - formate dehydrogenase, at 2,850,548 to 2,852,977 _RS32440 Position (kb) 2849 2850 2851Strain fitness (log2 ratio) -1 0 1 2 3 4at 2848.854 kb on + strandat 2848.951 kb on - strandat 2848.951 kb on - strandat 2849.018 kb on + strandat 2849.018 kb on + strandat 2849.076 kb on - strand, within BPHYT_RS32430at 2849.266 kb on - strand, within BPHYT_RS32430at 2849.348 kb on + strand, within BPHYT_RS32430at 2849.348 kb on + strand, within BPHYT_RS32430at 2849.348 kb on + strand, within BPHYT_RS32430at 2849.349 kb on - strand, within BPHYT_RS32430at 2849.349 kb on - strand, within BPHYT_RS32430at 2849.349 kb on - strand, within BPHYT_RS32430at 2849.470 kb on - strand, within BPHYT_RS32430at 2849.542 kb on - strand, within BPHYT_RS32430at 2849.542 kb on - strand, within BPHYT_RS32430at 2849.642 kb on + strandat 2849.713 kb on - strand, within BPHYT_RS32435at 2850.066 kb on + strand, within BPHYT_RS32435at 2850.067 kb on - strand, within BPHYT_RS32435at 2850.346 kb on - strand, within BPHYT_RS32435at 2850.549 kb on + strandat 2850.757 kb on + strandat 2850.758 kb on - strandat 2850.913 kb on - strand, within BPHYT_RS32440at 2850.913 kb on - strand, within BPHYT_RS32440at 2851.029 kb on + strand, within BPHYT_RS32440at 2851.057 kb on - strand, within BPHYT_RS32440at 2851.272 kb on + strand, within BPHYT_RS32440at 2851.272 kb on + strand, within BPHYT_RS32440at 2851.483 kb on + strand, within BPHYT_RS32440at 2851.484 kb on - strand, within BPHYT_RS32440

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 0.1
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2,848,854 + +0.5
2,848,951 - +0.4
2,848,951 - -0.0
2,849,018 + -1.1
2,849,018 + -0.3
2,849,076 - BPHYT_RS32430 0.17 -0.6
2,849,266 - BPHYT_RS32430 0.46 -0.3
2,849,348 + BPHYT_RS32430 0.59 +0.3
2,849,348 + BPHYT_RS32430 0.59 +3.7
2,849,348 + BPHYT_RS32430 0.59 -1.0
2,849,349 - BPHYT_RS32430 0.59 +0.2
2,849,349 - BPHYT_RS32430 0.59 -0.1
2,849,349 - BPHYT_RS32430 0.59 +0.1
2,849,470 - BPHYT_RS32430 0.77 +0.0
2,849,542 - BPHYT_RS32430 0.88 +0.7
2,849,542 - BPHYT_RS32430 0.88 +0.0
2,849,642 + -0.7
2,849,713 - BPHYT_RS32435 0.11 +2.2
2,850,066 + BPHYT_RS32435 0.49 +0.9
2,850,067 - BPHYT_RS32435 0.49 -0.3
2,850,346 - BPHYT_RS32435 0.79 +0.4
2,850,549 + +4.2
2,850,757 + -1.0
2,850,758 - +0.2
2,850,913 - BPHYT_RS32440 0.15 +0.5
2,850,913 - BPHYT_RS32440 0.15 +1.7
2,851,029 + BPHYT_RS32440 0.20 +0.2
2,851,057 - BPHYT_RS32440 0.21 +0.2
2,851,272 + BPHYT_RS32440 0.30 -0.2
2,851,272 + BPHYT_RS32440 0.30 -0.4
2,851,483 + BPHYT_RS32440 0.38 -1.7
2,851,484 - BPHYT_RS32440 0.39 -0.3

Or see this region's nucleotide sequence