Experiment: 36hr PHYTO8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26580 and BPHYT_RS26585 are separated by 447 nucleotides BPHYT_RS26585 and BPHYT_RS26590 are separated by 92 nucleotides BPHYT_RS26590 and BPHYT_RS26595 are separated by 94 nucleotides
BPHYT_RS26580: BPHYT_RS26580 - hypothetical protein, at 1,531,646 to 1,532,545
_RS26580
BPHYT_RS26585: BPHYT_RS26585 - trehalase, at 1,532,993 to 1,534,666
_RS26585
BPHYT_RS26590: BPHYT_RS26590 - DNA-3-methyladenine glycosylase, at 1,534,759 to 1,535,379
_RS26590
BPHYT_RS26595: BPHYT_RS26595 - carbonic anhydrase, at 1,535,474 to 1,536,211
_RS26595
Position (kb)
1532
1533
1534
1535 Strain fitness (log2 ratio)
-2
-1
0
1 at 1532.630 kb on - strand at 1532.694 kb on + strand at 1532.738 kb on + strand at 1532.738 kb on + strand at 1532.822 kb on + strand at 1532.822 kb on + strand at 1532.822 kb on + strand at 1533.347 kb on + strand, within BPHYT_RS26585 at 1533.348 kb on - strand, within BPHYT_RS26585 at 1533.539 kb on + strand, within BPHYT_RS26585 at 1533.774 kb on - strand, within BPHYT_RS26585 at 1533.954 kb on - strand, within BPHYT_RS26585 at 1534.187 kb on + strand, within BPHYT_RS26585 at 1534.208 kb on + strand, within BPHYT_RS26585 at 1534.374 kb on - strand, within BPHYT_RS26585 at 1534.514 kb on + strand at 1534.514 kb on + strand at 1534.938 kb on + strand, within BPHYT_RS26590 at 1534.957 kb on + strand, within BPHYT_RS26590 at 1534.957 kb on + strand, within BPHYT_RS26590 at 1534.958 kb on - strand, within BPHYT_RS26590 at 1535.091 kb on + strand, within BPHYT_RS26590 at 1535.092 kb on - strand, within BPHYT_RS26590 at 1535.285 kb on - strand, within BPHYT_RS26590 at 1535.285 kb on - strand, within BPHYT_RS26590 at 1535.508 kb on + strand at 1535.547 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 0.1 remove 1,532,630 - -0.2 1,532,694 + -0.4 1,532,738 + +0.8 1,532,738 + -1.0 1,532,822 + -0.6 1,532,822 + +0.2 1,532,822 + +0.1 1,533,347 + BPHYT_RS26585 0.21 +1.0 1,533,348 - BPHYT_RS26585 0.21 -0.5 1,533,539 + BPHYT_RS26585 0.33 +0.0 1,533,774 - BPHYT_RS26585 0.47 -1.7 1,533,954 - BPHYT_RS26585 0.57 +0.4 1,534,187 + BPHYT_RS26585 0.71 -2.5 1,534,208 + BPHYT_RS26585 0.73 -0.2 1,534,374 - BPHYT_RS26585 0.82 -0.0 1,534,514 + -0.3 1,534,514 + -0.2 1,534,938 + BPHYT_RS26590 0.29 -1.5 1,534,957 + BPHYT_RS26590 0.32 +0.9 1,534,957 + BPHYT_RS26590 0.32 -0.2 1,534,958 - BPHYT_RS26590 0.32 -0.0 1,535,091 + BPHYT_RS26590 0.53 -1.0 1,535,092 - BPHYT_RS26590 0.54 +1.4 1,535,285 - BPHYT_RS26590 0.85 +1.1 1,535,285 - BPHYT_RS26590 0.85 +0.3 1,535,508 + +0.7 1,535,547 + -1.9
Or see this region's nucleotide sequence