Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22520

Experiment: 36hr PHYTO8 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22515 and BPHYT_RS22520 are separated by 13 nucleotidesBPHYT_RS22520 and BPHYT_RS22525 are separated by 375 nucleotides BPHYT_RS22515: BPHYT_RS22515 - multidrug efflux RND transporter permease subunit, at 609,884 to 613,069 _RS22515 BPHYT_RS22520: BPHYT_RS22520 - RND transporter, at 613,083 to 614,684 _RS22520 BPHYT_RS22525: BPHYT_RS22525 - dioxygenase, at 615,060 to 615,899 _RS22525 Position (kb) 613 614 615Strain fitness (log2 ratio) -2 -1 0 1at 612.209 kb on + strand, within BPHYT_RS22515at 612.360 kb on - strand, within BPHYT_RS22515at 612.368 kb on + strand, within BPHYT_RS22515at 612.371 kb on - strand, within BPHYT_RS22515at 612.498 kb on - strand, within BPHYT_RS22515at 612.582 kb on - strand, within BPHYT_RS22515at 612.980 kb on + strandat 612.981 kb on - strandat 613.043 kb on + strandat 613.720 kb on - strand, within BPHYT_RS22520at 613.759 kb on - strand, within BPHYT_RS22520at 613.759 kb on - strand, within BPHYT_RS22520at 614.445 kb on + strand, within BPHYT_RS22520at 614.467 kb on - strand, within BPHYT_RS22520at 614.467 kb on - strand, within BPHYT_RS22520at 614.790 kb on + strandat 614.814 kb on - strandat 615.293 kb on + strand, within BPHYT_RS22525at 615.337 kb on - strand, within BPHYT_RS22525at 615.348 kb on - strand, within BPHYT_RS22525at 615.384 kb on + strand, within BPHYT_RS22525at 615.432 kb on + strand, within BPHYT_RS22525

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 0.1
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612,209 + BPHYT_RS22515 0.73 -0.0
612,360 - BPHYT_RS22515 0.78 -0.4
612,368 + BPHYT_RS22515 0.78 -0.3
612,371 - BPHYT_RS22515 0.78 -0.1
612,498 - BPHYT_RS22515 0.82 -0.2
612,582 - BPHYT_RS22515 0.85 +0.7
612,980 + -2.4
612,981 - +1.0
613,043 + -0.0
613,720 - BPHYT_RS22520 0.40 -1.3
613,759 - BPHYT_RS22520 0.42 +1.1
613,759 - BPHYT_RS22520 0.42 +0.7
614,445 + BPHYT_RS22520 0.85 -0.5
614,467 - BPHYT_RS22520 0.86 -1.9
614,467 - BPHYT_RS22520 0.86 +0.3
614,790 + -0.1
614,814 - +0.7
615,293 + BPHYT_RS22525 0.28 -0.3
615,337 - BPHYT_RS22525 0.33 -0.8
615,348 - BPHYT_RS22525 0.34 +0.9
615,384 + BPHYT_RS22525 0.39 +0.3
615,432 + BPHYT_RS22525 0.44 +0.6

Or see this region's nucleotide sequence