Experiment: 36hr PHYTO8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17600 and BPHYT_RS17605 are separated by 393 nucleotides BPHYT_RS17605 and BPHYT_RS17610 are separated by 19 nucleotides BPHYT_RS17610 and BPHYT_RS17615 are separated by 70 nucleotides BPHYT_RS17615 and BPHYT_RS17620 are separated by 95 nucleotides
BPHYT_RS17600: BPHYT_RS17600 - methylase, at 3,983,822 to 3,984,946
_RS17600
BPHYT_RS17605: BPHYT_RS17605 - tRNA-Thr, at 3,985,340 to 3,985,415
_RS17605
BPHYT_RS17610: BPHYT_RS17610 - peptidase, at 3,985,435 to 3,985,944
_RS17610
BPHYT_RS17615: BPHYT_RS17615 - Glutathione S-transferase domain, at 3,986,015 to 3,986,626
_RS17615
BPHYT_RS17620: BPHYT_RS17620 - cytochrome C, at 3,986,722 to 3,987,477
_RS17620
Position (kb)
3985
3986 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3984.552 kb on + strand, within BPHYT_RS17600 at 3984.553 kb on - strand, within BPHYT_RS17600 at 3984.607 kb on - strand, within BPHYT_RS17600 at 3985.023 kb on + strand at 3985.024 kb on - strand at 3985.024 kb on - strand at 3985.024 kb on - strand at 3985.024 kb on - strand at 3985.024 kb on - strand at 3985.024 kb on - strand at 3985.024 kb on - strand at 3985.050 kb on + strand at 3985.053 kb on + strand at 3985.053 kb on + strand at 3985.054 kb on - strand at 3985.176 kb on + strand at 3985.274 kb on + strand at 3985.275 kb on - strand at 3985.332 kb on - strand at 3985.332 kb on - strand at 3985.335 kb on - strand at 3985.441 kb on + strand at 3985.689 kb on + strand, within BPHYT_RS17610 at 3985.901 kb on + strand at 3985.901 kb on + strand at 3985.902 kb on - strand at 3985.919 kb on + strand at 3986.724 kb on - strand at 3986.724 kb on - strand at 3986.731 kb on - strand at 3986.888 kb on - strand, within BPHYT_RS17620
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 0.1 remove 3,984,552 + BPHYT_RS17600 0.65 -0.2 3,984,553 - BPHYT_RS17600 0.65 -1.4 3,984,607 - BPHYT_RS17600 0.70 -1.2 3,985,023 + +0.3 3,985,024 - -0.1 3,985,024 - -1.2 3,985,024 - -0.1 3,985,024 - +2.1 3,985,024 - -0.3 3,985,024 - -2.3 3,985,024 - -0.3 3,985,050 + -1.2 3,985,053 + -0.2 3,985,053 + -0.1 3,985,054 - -1.5 3,985,176 + -0.4 3,985,274 + +0.8 3,985,275 - -0.8 3,985,332 - -0.0 3,985,332 - -0.1 3,985,335 - -0.2 3,985,441 + +1.5 3,985,689 + BPHYT_RS17610 0.50 +0.2 3,985,901 + +0.5 3,985,901 + +0.1 3,985,902 - +1.2 3,985,919 + +0.5 3,986,724 - -0.0 3,986,724 - -0.3 3,986,731 - -1.3 3,986,888 - BPHYT_RS17620 0.22 -1.7
Or see this region's nucleotide sequence