Experiment: 36hr PHYTO8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17340 and BPHYT_RS17345 are separated by 125 nucleotides BPHYT_RS17345 and BPHYT_RS17350 are separated by 50 nucleotides BPHYT_RS17350 and BPHYT_RS17355 are separated by 2 nucleotides
BPHYT_RS17340: BPHYT_RS17340 - 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase PaaZ (EC:1.2.1.91) (from data) , at 3,930,706 to 3,932,406
_RS17340
BPHYT_RS17345: BPHYT_RS17345 - bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (from data) , at 3,932,532 to 3,933,734
_RS17345
BPHYT_RS17350: BPHYT_RS17350 - enoyl-CoA hydratase, at 3,933,785 to 3,934,576
_RS17350
BPHYT_RS17355: BPHYT_RS17355 - phenylacetic acid degradation protein, at 3,934,579 to 3,935,040
_RS17355
Position (kb)
3932
3933
3934 Strain fitness (log2 ratio)
-1
0
1 at 3931.717 kb on + strand, within BPHYT_RS17340 at 3931.718 kb on - strand, within BPHYT_RS17340 at 3931.753 kb on + strand, within BPHYT_RS17340 at 3931.753 kb on + strand, within BPHYT_RS17340 at 3931.754 kb on - strand, within BPHYT_RS17340 at 3931.989 kb on - strand, within BPHYT_RS17340 at 3932.155 kb on + strand, within BPHYT_RS17340 at 3932.413 kb on + strand at 3932.413 kb on + strand at 3932.413 kb on + strand at 3932.414 kb on - strand at 3932.414 kb on - strand at 3932.436 kb on + strand at 3932.476 kb on + strand at 3932.476 kb on + strand at 3932.477 kb on - strand at 3932.477 kb on - strand at 3932.677 kb on + strand, within BPHYT_RS17345 at 3932.677 kb on + strand, within BPHYT_RS17345 at 3932.691 kb on + strand, within BPHYT_RS17345 at 3932.691 kb on + strand, within BPHYT_RS17345 at 3932.691 kb on + strand, within BPHYT_RS17345 at 3932.691 kb on + strand, within BPHYT_RS17345 at 3932.924 kb on + strand, within BPHYT_RS17345 at 3932.924 kb on + strand, within BPHYT_RS17345 at 3932.924 kb on + strand, within BPHYT_RS17345 at 3932.948 kb on + strand, within BPHYT_RS17345 at 3932.949 kb on - strand, within BPHYT_RS17345 at 3932.952 kb on + strand, within BPHYT_RS17345 at 3932.953 kb on - strand, within BPHYT_RS17345 at 3933.004 kb on - strand, within BPHYT_RS17345 at 3933.242 kb on + strand, within BPHYT_RS17345 at 3933.534 kb on - strand, within BPHYT_RS17345 at 3933.735 kb on + strand at 3933.791 kb on + strand at 3933.791 kb on + strand at 3933.791 kb on + strand at 3933.791 kb on + strand at 3933.792 kb on - strand at 3933.846 kb on + strand at 3934.517 kb on + strand at 3934.652 kb on - strand, within BPHYT_RS17355 at 3934.700 kb on + strand, within BPHYT_RS17355
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 0.1 remove 3,931,717 + BPHYT_RS17340 0.59 -1.1 3,931,718 - BPHYT_RS17340 0.59 -0.3 3,931,753 + BPHYT_RS17340 0.62 +0.8 3,931,753 + BPHYT_RS17340 0.62 -1.2 3,931,754 - BPHYT_RS17340 0.62 -0.3 3,931,989 - BPHYT_RS17340 0.75 -1.2 3,932,155 + BPHYT_RS17340 0.85 -0.6 3,932,413 + -1.1 3,932,413 + +0.4 3,932,413 + -0.3 3,932,414 - -0.2 3,932,414 - -0.4 3,932,436 + +0.1 3,932,476 + -1.3 3,932,476 + -1.1 3,932,477 - +0.5 3,932,477 - -0.3 3,932,677 + BPHYT_RS17345 0.12 -0.3 3,932,677 + BPHYT_RS17345 0.12 -0.7 3,932,691 + BPHYT_RS17345 0.13 -0.3 3,932,691 + BPHYT_RS17345 0.13 -1.3 3,932,691 + BPHYT_RS17345 0.13 +0.6 3,932,691 + BPHYT_RS17345 0.13 -0.3 3,932,924 + BPHYT_RS17345 0.33 -0.6 3,932,924 + BPHYT_RS17345 0.33 -0.6 3,932,924 + BPHYT_RS17345 0.33 -0.4 3,932,948 + BPHYT_RS17345 0.35 -0.3 3,932,949 - BPHYT_RS17345 0.35 +0.5 3,932,952 + BPHYT_RS17345 0.35 -0.4 3,932,953 - BPHYT_RS17345 0.35 -0.0 3,933,004 - BPHYT_RS17345 0.39 +0.5 3,933,242 + BPHYT_RS17345 0.59 +0.5 3,933,534 - BPHYT_RS17345 0.83 +0.4 3,933,735 + -0.3 3,933,791 + -0.0 3,933,791 + -1.2 3,933,791 + -1.0 3,933,791 + +0.2 3,933,792 - +0.3 3,933,846 + +1.0 3,934,517 + -1.0 3,934,652 - BPHYT_RS17355 0.16 -0.7 3,934,700 + BPHYT_RS17355 0.26 +0.5
Or see this region's nucleotide sequence