Experiment: 36hr PHYTO8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14345 and BPHYT_RS14350 are separated by 69 nucleotides BPHYT_RS14350 and BPHYT_RS14355 are separated by 15 nucleotides BPHYT_RS14355 and BPHYT_RS14360 overlap by 4 nucleotides BPHYT_RS14360 and BPHYT_RS14365 overlap by 14 nucleotides
BPHYT_RS14345: BPHYT_RS14345 - beta-hexosaminidase, at 3,275,455 to 3,276,483
_RS14345
BPHYT_RS14350: BPHYT_RS14350 - holo-[acyl-carrier-protein] synthase, at 3,276,553 to 3,276,975
_RS14350
BPHYT_RS14355: BPHYT_RS14355 - pyridoxine 5'-phosphate synthase, at 3,276,991 to 3,277,755
_RS14355
BPHYT_RS14360: BPHYT_RS14360 - DNA repair protein RecO, at 3,277,752 to 3,278,681
_RS14360
BPHYT_RS14365: BPHYT_RS14365 - GTPase Era, at 3,278,668 to 3,279,531
_RS14365
Position (kb)
3276
3277
3278 Strain fitness (log2 ratio)
-1
0
1
2 at 3276.093 kb on - strand, within BPHYT_RS14345 at 3276.161 kb on + strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.549 kb on - strand at 3277.756 kb on - strand at 3277.811 kb on - strand at 3277.870 kb on + strand, within BPHYT_RS14360 at 3277.871 kb on - strand, within BPHYT_RS14360 at 3277.975 kb on + strand, within BPHYT_RS14360 at 3278.122 kb on + strand, within BPHYT_RS14360 at 3278.122 kb on + strand, within BPHYT_RS14360 at 3278.122 kb on + strand, within BPHYT_RS14360 at 3278.123 kb on - strand, within BPHYT_RS14360 at 3278.123 kb on - strand, within BPHYT_RS14360 at 3278.188 kb on + strand, within BPHYT_RS14360 at 3278.422 kb on + strand, within BPHYT_RS14360 at 3278.423 kb on - strand at 3278.423 kb on - strand, within BPHYT_RS14360 at 3278.426 kb on + strand, within BPHYT_RS14360 at 3278.426 kb on + strand, within BPHYT_RS14360 at 3278.427 kb on - strand, within BPHYT_RS14360 at 3278.427 kb on - strand, within BPHYT_RS14360 at 3278.570 kb on + strand, within BPHYT_RS14360 at 3278.570 kb on + strand, within BPHYT_RS14360 at 3278.571 kb on - strand, within BPHYT_RS14360 at 3278.682 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 0.1 remove 3,276,093 - BPHYT_RS14345 0.62 +1.0 3,276,161 + BPHYT_RS14345 0.69 -0.7 3,276,162 - BPHYT_RS14345 0.69 -0.7 3,276,162 - BPHYT_RS14345 0.69 -1.5 3,276,162 - BPHYT_RS14345 0.69 +1.6 3,276,549 - +1.2 3,277,756 - +1.6 3,277,811 - +0.2 3,277,870 + BPHYT_RS14360 0.13 -0.8 3,277,871 - BPHYT_RS14360 0.13 +1.6 3,277,975 + BPHYT_RS14360 0.24 +1.0 3,278,122 + BPHYT_RS14360 0.40 -0.1 3,278,122 + BPHYT_RS14360 0.40 -1.7 3,278,122 + BPHYT_RS14360 0.40 +0.0 3,278,123 - BPHYT_RS14360 0.40 +1.4 3,278,123 - BPHYT_RS14360 0.40 -0.8 3,278,188 + BPHYT_RS14360 0.47 +1.1 3,278,422 + BPHYT_RS14360 0.72 -0.0 3,278,423 - -0.8 3,278,423 - BPHYT_RS14360 0.72 -1.2 3,278,426 + BPHYT_RS14360 0.72 -1.5 3,278,426 + BPHYT_RS14360 0.72 +0.4 3,278,427 - BPHYT_RS14360 0.73 +2.6 3,278,427 - BPHYT_RS14360 0.73 -0.6 3,278,570 + BPHYT_RS14360 0.88 -0.3 3,278,570 + BPHYT_RS14360 0.88 +0.4 3,278,571 - BPHYT_RS14360 0.88 +1.6 3,278,682 - +2.0
Or see this region's nucleotide sequence