Experiment: 36hr PHYTO8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10710 and BPHYT_RS10715 are separated by 236 nucleotides BPHYT_RS10715 and BPHYT_RS10720 are separated by 67 nucleotides BPHYT_RS10720 and BPHYT_RS10725 are separated by 18 nucleotides BPHYT_RS10725 and BPHYT_RS10730 overlap by 4 nucleotides
BPHYT_RS10710: BPHYT_RS10710 - sensor histidine kinase, at 2,437,256 to 2,438,776
_RS10710
BPHYT_RS10715: BPHYT_RS10715 - acyl-CoA dehydrogenase, at 2,439,013 to 2,440,191
_RS10715
BPHYT_RS10720: BPHYT_RS10720 - hypothetical protein, at 2,440,259 to 2,440,636
_RS10720
BPHYT_RS10725: BPHYT_RS10725 - polyketide cyclase, at 2,440,655 to 2,441,170
_RS10725
BPHYT_RS10730: BPHYT_RS10730 - Glu-tRNA amidotransferase, at 2,441,167 to 2,442,291
_RS10730
Position (kb)
2439
2440
2441 Strain fitness (log2 ratio)
-1
0
1 at 2438.807 kb on + strand at 2438.927 kb on + strand at 2439.333 kb on + strand, within BPHYT_RS10715 at 2439.698 kb on - strand, within BPHYT_RS10715 at 2440.136 kb on - strand at 2440.136 kb on - strand at 2440.189 kb on + strand at 2440.217 kb on - strand at 2440.475 kb on + strand, within BPHYT_RS10720 at 2440.554 kb on - strand, within BPHYT_RS10720 at 2440.748 kb on - strand, within BPHYT_RS10725 at 2441.018 kb on + strand, within BPHYT_RS10725
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 0.1 remove 2,438,807 + -0.1 2,438,927 + +0.7 2,439,333 + BPHYT_RS10715 0.27 -1.0 2,439,698 - BPHYT_RS10715 0.58 -0.1 2,440,136 - +0.0 2,440,136 - -0.4 2,440,189 + -0.2 2,440,217 - -1.7 2,440,475 + BPHYT_RS10720 0.57 +0.3 2,440,554 - BPHYT_RS10720 0.78 -0.7 2,440,748 - BPHYT_RS10725 0.18 +1.5 2,441,018 + BPHYT_RS10725 0.70 +0.1
Or see this region's nucleotide sequence