Experiment: 36hr PHYTO8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05135 and BPHYT_RS05140 are separated by 209 nucleotides BPHYT_RS05140 and BPHYT_RS05145 are separated by 124 nucleotides
BPHYT_RS05135: BPHYT_RS05135 - carboxylesterase, at 1,184,304 to 1,185,119
_RS05135
BPHYT_RS05140: BPHYT_RS05140 - manganese transporter, at 1,185,329 to 1,186,654
_RS05140
BPHYT_RS05145: BPHYT_RS05145 - hypothetical protein, at 1,186,779 to 1,187,759
_RS05145
Position (kb)
1185
1186
1187 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1184.370 kb on - strand at 1184.749 kb on + strand, within BPHYT_RS05135 at 1184.749 kb on + strand, within BPHYT_RS05135 at 1184.920 kb on + strand, within BPHYT_RS05135 at 1184.978 kb on - strand, within BPHYT_RS05135 at 1185.076 kb on - strand at 1185.079 kb on + strand at 1185.079 kb on + strand at 1185.080 kb on - strand at 1185.080 kb on - strand at 1185.166 kb on - strand at 1185.280 kb on - strand at 1185.280 kb on - strand at 1185.628 kb on + strand, within BPHYT_RS05140 at 1186.099 kb on - strand, within BPHYT_RS05140 at 1186.103 kb on + strand, within BPHYT_RS05140 at 1186.183 kb on - strand, within BPHYT_RS05140 at 1186.350 kb on - strand, within BPHYT_RS05140 at 1186.451 kb on - strand, within BPHYT_RS05140 at 1186.453 kb on - strand, within BPHYT_RS05140 at 1186.879 kb on + strand, within BPHYT_RS05145 at 1186.879 kb on + strand, within BPHYT_RS05145 at 1186.879 kb on + strand, within BPHYT_RS05145 at 1186.880 kb on - strand, within BPHYT_RS05145 at 1187.050 kb on + strand, within BPHYT_RS05145 at 1187.154 kb on - strand, within BPHYT_RS05145 at 1187.156 kb on - strand, within BPHYT_RS05145 at 1187.185 kb on + strand, within BPHYT_RS05145 at 1187.185 kb on + strand, within BPHYT_RS05145 at 1187.507 kb on - strand, within BPHYT_RS05145 at 1187.511 kb on - strand, within BPHYT_RS05145 at 1187.530 kb on + strand, within BPHYT_RS05145
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 0.1 remove 1,184,370 - -0.4 1,184,749 + BPHYT_RS05135 0.55 -1.2 1,184,749 + BPHYT_RS05135 0.55 -0.3 1,184,920 + BPHYT_RS05135 0.75 -0.9 1,184,978 - BPHYT_RS05135 0.83 -0.3 1,185,076 - -0.8 1,185,079 + -0.2 1,185,079 + -1.0 1,185,080 - -0.1 1,185,080 - -0.6 1,185,166 - -0.5 1,185,280 - +0.5 1,185,280 - +0.8 1,185,628 + BPHYT_RS05140 0.23 +0.2 1,186,099 - BPHYT_RS05140 0.58 -2.1 1,186,103 + BPHYT_RS05140 0.58 +0.5 1,186,183 - BPHYT_RS05140 0.64 +0.6 1,186,350 - BPHYT_RS05140 0.77 +0.2 1,186,451 - BPHYT_RS05140 0.85 -2.5 1,186,453 - BPHYT_RS05140 0.85 -1.2 1,186,879 + BPHYT_RS05145 0.10 +0.6 1,186,879 + BPHYT_RS05145 0.10 -1.1 1,186,879 + BPHYT_RS05145 0.10 -1.1 1,186,880 - BPHYT_RS05145 0.10 +0.3 1,187,050 + BPHYT_RS05145 0.28 -2.0 1,187,154 - BPHYT_RS05145 0.38 -1.2 1,187,156 - BPHYT_RS05145 0.38 +1.6 1,187,185 + BPHYT_RS05145 0.41 +1.0 1,187,185 + BPHYT_RS05145 0.41 -3.4 1,187,507 - BPHYT_RS05145 0.74 -2.4 1,187,511 - BPHYT_RS05145 0.75 +0.1 1,187,530 + BPHYT_RS05145 0.77 +0.3
Or see this region's nucleotide sequence