Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33025 and BPHYT_RS33030 are separated by 135 nucleotides BPHYT_RS33030 and BPHYT_RS33035 are separated by 70 nucleotides BPHYT_RS33035 and BPHYT_RS33040 are separated by 156 nucleotides BPHYT_RS33040 and BPHYT_RS33045 are separated by 395 nucleotides
BPHYT_RS33025: BPHYT_RS33025 - hypothetical protein, at 2,987,263 to 2,988,075
_RS33025
BPHYT_RS33030: BPHYT_RS33030 - hypothetical protein, at 2,988,211 to 2,988,975
_RS33030
BPHYT_RS33035: BPHYT_RS33035 - murein transglycosylase, at 2,989,046 to 2,990,065
_RS33035
BPHYT_RS33040: BPHYT_RS33040 - hypothetical protein, at 2,990,222 to 2,990,440
_RS33040
BPHYT_RS33045: BPHYT_RS33045 - response regulator receiver protein, at 2,990,836 to 2,991,213
_RS33045
Position (kb)
2989
2990
2991 Strain fitness (log2 ratio)
-2
-1
0
1 at 2988.614 kb on + strand, within BPHYT_RS33030 at 2989.325 kb on + strand, within BPHYT_RS33035 at 2989.561 kb on + strand, within BPHYT_RS33035 at 2989.760 kb on + strand, within BPHYT_RS33035 at 2990.009 kb on + strand at 2990.010 kb on - strand at 2990.010 kb on - strand at 2990.199 kb on + strand at 2990.330 kb on + strand, within BPHYT_RS33040 at 2990.337 kb on - strand, within BPHYT_RS33040 at 2990.397 kb on - strand, within BPHYT_RS33040 at 2990.461 kb on + strand at 2990.484 kb on - strand at 2990.570 kb on - strand at 2990.626 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 2,988,614 + BPHYT_RS33030 0.53 +0.7 2,989,325 + BPHYT_RS33035 0.27 -1.5 2,989,561 + BPHYT_RS33035 0.50 +1.0 2,989,760 + BPHYT_RS33035 0.70 -1.3 2,990,009 + +0.2 2,990,010 - -0.9 2,990,010 - -1.0 2,990,199 + -0.1 2,990,330 + BPHYT_RS33040 0.49 +0.5 2,990,337 - BPHYT_RS33040 0.53 -1.2 2,990,397 - BPHYT_RS33040 0.80 -0.0 2,990,461 + -1.2 2,990,484 - -0.0 2,990,570 - +0.1 2,990,626 + -1.8
Or see this region's nucleotide sequence