Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30150 and BPHYT_RS30155 are separated by 218 nucleotides BPHYT_RS30155 and BPHYT_RS30160 overlap by 44 nucleotides BPHYT_RS30160 and BPHYT_RS30165 overlap by 4 nucleotides
BPHYT_RS30150: BPHYT_RS30150 - methylcitrate synthase, at 2,317,995 to 2,319,164
_RS30150
BPHYT_RS30155: BPHYT_RS30155 - Fis family transcriptional regulator, at 2,319,383 to 2,321,374
_RS30155
BPHYT_RS30160: BPHYT_RS30160 - AraC family transcriptional regulator, at 2,321,331 to 2,321,759
_RS30160
BPHYT_RS30165: BPHYT_RS30165 - sarcosine oxidase subunit beta, at 2,321,756 to 2,322,925
_RS30165
Position (kb)
2319
2320
2321
2322 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2318.506 kb on + strand, within BPHYT_RS30150 at 2318.510 kb on + strand, within BPHYT_RS30150 at 2318.838 kb on + strand, within BPHYT_RS30150 at 2318.838 kb on + strand, within BPHYT_RS30150 at 2319.245 kb on + strand at 2319.341 kb on + strand at 2319.479 kb on + strand at 2319.480 kb on - strand at 2319.755 kb on + strand, within BPHYT_RS30155 at 2319.783 kb on - strand, within BPHYT_RS30155 at 2320.187 kb on - strand, within BPHYT_RS30155 at 2320.187 kb on - strand, within BPHYT_RS30155 at 2320.205 kb on + strand, within BPHYT_RS30155 at 2320.205 kb on + strand, within BPHYT_RS30155 at 2320.205 kb on + strand, within BPHYT_RS30155 at 2320.205 kb on + strand, within BPHYT_RS30155 at 2320.206 kb on - strand, within BPHYT_RS30155 at 2320.357 kb on + strand, within BPHYT_RS30155 at 2320.836 kb on - strand, within BPHYT_RS30155 at 2321.130 kb on + strand, within BPHYT_RS30155 at 2321.381 kb on + strand, within BPHYT_RS30160 at 2321.623 kb on + strand, within BPHYT_RS30160 at 2321.846 kb on - strand at 2321.976 kb on + strand, within BPHYT_RS30165 at 2322.162 kb on + strand, within BPHYT_RS30165 at 2322.217 kb on - strand, within BPHYT_RS30165
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 2,318,506 + BPHYT_RS30150 0.44 -0.5 2,318,510 + BPHYT_RS30150 0.44 +0.9 2,318,838 + BPHYT_RS30150 0.72 -2.4 2,318,838 + BPHYT_RS30150 0.72 +0.4 2,319,245 + +0.1 2,319,341 + -0.2 2,319,479 + -1.2 2,319,480 - -0.2 2,319,755 + BPHYT_RS30155 0.19 -0.3 2,319,783 - BPHYT_RS30155 0.20 -1.1 2,320,187 - BPHYT_RS30155 0.40 -0.2 2,320,187 - BPHYT_RS30155 0.40 -0.6 2,320,205 + BPHYT_RS30155 0.41 -2.1 2,320,205 + BPHYT_RS30155 0.41 -0.3 2,320,205 + BPHYT_RS30155 0.41 -1.2 2,320,205 + BPHYT_RS30155 0.41 -0.5 2,320,206 - BPHYT_RS30155 0.41 +0.5 2,320,357 + BPHYT_RS30155 0.49 +0.4 2,320,836 - BPHYT_RS30155 0.73 -0.2 2,321,130 + BPHYT_RS30155 0.88 -0.2 2,321,381 + BPHYT_RS30160 0.12 -3.0 2,321,623 + BPHYT_RS30160 0.68 -0.8 2,321,846 - +0.4 2,321,976 + BPHYT_RS30165 0.19 -0.1 2,322,162 + BPHYT_RS30165 0.35 -0.5 2,322,217 - BPHYT_RS30165 0.39 +0.4
Or see this region's nucleotide sequence