Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29805 and BPHYT_RS29810 are separated by 13 nucleotides BPHYT_RS29810 and BPHYT_RS29815 are separated by 43 nucleotides BPHYT_RS29815 and BPHYT_RS29820 are separated by 61 nucleotides BPHYT_RS29820 and BPHYT_RS29825 are separated by 14 nucleotides
BPHYT_RS29805: BPHYT_RS29805 - lysine transporter LysE, at 2,239,478 to 2,240,098
_RS29805
BPHYT_RS29810: BPHYT_RS29810 - hexapeptide transferase, at 2,240,112 to 2,240,531
_RS29810
BPHYT_RS29815: BPHYT_RS29815 - UDP-N-acetyl-D-mannosamine transferase, at 2,240,575 to 2,241,309
_RS29815
BPHYT_RS29820: BPHYT_RS29820 - hypothetical protein, at 2,241,371 to 2,242,168
_RS29820
BPHYT_RS29825: BPHYT_RS29825 - glycosyl transferase family 1, at 2,242,183 to 2,243,337
_RS29825
Position (kb)
2240
2241
2242 Strain fitness (log2 ratio)
-1
0
1 at 2239.970 kb on + strand, within BPHYT_RS29805 at 2239.971 kb on - strand, within BPHYT_RS29805 at 2239.989 kb on - strand, within BPHYT_RS29805 at 2240.314 kb on - strand, within BPHYT_RS29810 at 2240.546 kb on + strand at 2240.547 kb on - strand at 2240.996 kb on + strand, within BPHYT_RS29815 at 2241.120 kb on + strand, within BPHYT_RS29815 at 2241.661 kb on + strand, within BPHYT_RS29820 at 2241.826 kb on - strand, within BPHYT_RS29820 at 2242.051 kb on - strand, within BPHYT_RS29820 at 2242.293 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 2,239,970 + BPHYT_RS29805 0.79 -1.2 2,239,971 - BPHYT_RS29805 0.79 +1.5 2,239,989 - BPHYT_RS29805 0.82 -0.1 2,240,314 - BPHYT_RS29810 0.48 -1.7 2,240,546 + +1.1 2,240,547 - -0.2 2,240,996 + BPHYT_RS29815 0.57 -1.3 2,241,120 + BPHYT_RS29815 0.74 -1.3 2,241,661 + BPHYT_RS29820 0.36 -1.1 2,241,826 - BPHYT_RS29820 0.57 -1.1 2,242,051 - BPHYT_RS29820 0.85 -0.9 2,242,293 + +1.0
Or see this region's nucleotide sequence