Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29675 and BPHYT_RS29680 are separated by 86 nucleotides BPHYT_RS29680 and BPHYT_RS29685 are separated by 171 nucleotides BPHYT_RS29685 and BPHYT_RS29690 are separated by 136 nucleotides BPHYT_RS29690 and BPHYT_RS29695 are separated by 23 nucleotides
BPHYT_RS29675: BPHYT_RS29675 - flagellar motor protein MotB, at 2,210,332 to 2,211,321
_RS29675
BPHYT_RS29680: BPHYT_RS29680 - hypothetical protein, at 2,211,408 to 2,212,091
_RS29680
BPHYT_RS29685: BPHYT_RS29685 - hypothetical protein, at 2,212,263 to 2,212,550
_RS29685
BPHYT_RS29690: BPHYT_RS29690 - exodeoxyribonuclease III, at 2,212,687 to 2,213,499
_RS29690
BPHYT_RS29695: BPHYT_RS29695 - hypothetical protein, at 2,213,523 to 2,213,741
_RS29695
Position (kb)
2212
2213 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2211.467 kb on - strand at 2211.698 kb on + strand, within BPHYT_RS29680 at 2211.723 kb on + strand, within BPHYT_RS29680 at 2211.723 kb on + strand, within BPHYT_RS29680 at 2211.723 kb on + strand, within BPHYT_RS29680 at 2211.769 kb on - strand, within BPHYT_RS29680 at 2212.044 kb on - strand at 2212.164 kb on + strand at 2212.165 kb on - strand at 2212.284 kb on - strand at 2213.018 kb on + strand, within BPHYT_RS29690 at 2213.019 kb on - strand, within BPHYT_RS29690 at 2213.084 kb on + strand, within BPHYT_RS29690 at 2213.086 kb on + strand, within BPHYT_RS29690 at 2213.166 kb on + strand, within BPHYT_RS29690 at 2213.174 kb on + strand, within BPHYT_RS29690
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 2,211,467 - -0.4 2,211,698 + BPHYT_RS29680 0.42 -0.2 2,211,723 + BPHYT_RS29680 0.46 -1.0 2,211,723 + BPHYT_RS29680 0.46 +0.7 2,211,723 + BPHYT_RS29680 0.46 -0.5 2,211,769 - BPHYT_RS29680 0.53 +0.1 2,212,044 - +0.5 2,212,164 + -0.4 2,212,165 - -1.3 2,212,284 - +1.2 2,213,018 + BPHYT_RS29690 0.41 +0.1 2,213,019 - BPHYT_RS29690 0.41 -0.2 2,213,084 + BPHYT_RS29690 0.49 +0.4 2,213,086 + BPHYT_RS29690 0.49 +3.7 2,213,166 + BPHYT_RS29690 0.59 -0.2 2,213,174 + BPHYT_RS29690 0.60 +0.3
Or see this region's nucleotide sequence