Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27965 and BPHYT_RS27970 overlap by 4 nucleotides BPHYT_RS27970 and BPHYT_RS27975 overlap by 4 nucleotides BPHYT_RS27975 and BPHYT_RS27980 are separated by 67 nucleotides
BPHYT_RS27965: BPHYT_RS27965 - hypothetical protein, at 1,839,363 to 1,839,557
_RS27965
BPHYT_RS27970: BPHYT_RS27970 - sugar ABC transporter permease, at 1,839,554 to 1,840,507
_RS27970
BPHYT_RS27975: BPHYT_RS27975 - ABC transporter permease, at 1,840,504 to 1,841,460
_RS27975
BPHYT_RS27980: BPHYT_RS27980 - ABC transporter substrate-binding protein, at 1,841,528 to 1,842,859
_RS27980
Position (kb) 1840
1841
1842 Strain fitness (log2 ratio) -1
0
1
2 at 1839.619 kb on + strand at 1839.620 kb on - strand at 1839.997 kb on - strand, within BPHYT_RS27970 at 1840.311 kb on + strand, within BPHYT_RS27970 at 1840.312 kb on - strand, within BPHYT_RS27970 at 1840.312 kb on - strand, within BPHYT_RS27970 at 1840.821 kb on - strand, within BPHYT_RS27975 at 1841.783 kb on + strand, within BPHYT_RS27980 at 1842.151 kb on - strand, within BPHYT_RS27980 at 1842.257 kb on + strand, within BPHYT_RS27980
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 1,839,619 + +0.5 1,839,620 - +0.6 1,839,997 - BPHYT_RS27970 0.46 -1.4 1,840,311 + BPHYT_RS27970 0.79 +1.8 1,840,312 - BPHYT_RS27970 0.79 +0.0 1,840,312 - BPHYT_RS27970 0.79 -0.6 1,840,821 - BPHYT_RS27975 0.33 -1.2 1,841,783 + BPHYT_RS27980 0.19 +1.4 1,842,151 - BPHYT_RS27980 0.47 -0.5 1,842,257 + BPHYT_RS27980 0.55 +0.5
Or see this region's nucleotide sequence