Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27640

Experiment: 36hr PHYTO8 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27635 and BPHYT_RS27640 are separated by 431 nucleotidesBPHYT_RS27640 and BPHYT_RS27645 are separated by 467 nucleotidesBPHYT_RS27645 and BPHYT_RS27650 are separated by 58 nucleotides BPHYT_RS27635: BPHYT_RS27635 - alcohol dehydrogenase, at 1,767,832 to 1,768,872 _RS27635 BPHYT_RS27640: BPHYT_RS27640 - acetoacetyl-CoA reductase, at 1,769,304 to 1,770,050 _RS27640 BPHYT_RS27645: BPHYT_RS27645 - universal stress protein UspA, at 1,770,518 to 1,770,976 _RS27645 BPHYT_RS27650: BPHYT_RS27650 - hypothetical protein, at 1,771,035 to 1,771,382 _RS27650 Position (kb) 1769 1770 1771Strain fitness (log2 ratio) -2 -1 0 1 2at 1768.509 kb on - strand, within BPHYT_RS27635at 1768.509 kb on - strand, within BPHYT_RS27635at 1768.705 kb on - strand, within BPHYT_RS27635at 1768.856 kb on + strandat 1769.230 kb on + strandat 1769.422 kb on - strand, within BPHYT_RS27640at 1769.591 kb on - strand, within BPHYT_RS27640at 1769.961 kb on + strand, within BPHYT_RS27640at 1770.050 kb on + strandat 1770.051 kb on - strandat 1770.451 kb on - strandat 1770.505 kb on - strandat 1770.981 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10
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1,768,509 - BPHYT_RS27635 0.65 -2.0
1,768,509 - BPHYT_RS27635 0.65 +1.6
1,768,705 - BPHYT_RS27635 0.84 -0.2
1,768,856 + +0.6
1,769,230 + -0.5
1,769,422 - BPHYT_RS27640 0.16 -1.5
1,769,591 - BPHYT_RS27640 0.38 +2.4
1,769,961 + BPHYT_RS27640 0.88 -0.6
1,770,050 + +0.3
1,770,051 - -0.1
1,770,451 - +0.4
1,770,505 - +0.8
1,770,981 - +0.9

Or see this region's nucleotide sequence