Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25115

Experiment: 36hr PHYTO8 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25105 and BPHYT_RS25110 are separated by 28 nucleotidesBPHYT_RS25110 and BPHYT_RS25115 overlap by 4 nucleotidesBPHYT_RS25115 and BPHYT_RS25120 overlap by 11 nucleotidesBPHYT_RS25120 and BPHYT_RS25125 are separated by 57 nucleotides BPHYT_RS25105: BPHYT_RS25105 - sarcosine oxidase subunit beta, at 1,205,073 to 1,206,317 _RS25105 BPHYT_RS25110: BPHYT_RS25110 - sarcosine oxidase subunit delta, at 1,206,346 to 1,206,684 _RS25110 BPHYT_RS25115: BPHYT_RS25115 - sarcosine oxidase subunit alpha, at 1,206,681 to 1,209,683 _RS25115 BPHYT_RS25120: BPHYT_RS25120 - sarcosine oxidase subunit gamma, at 1,209,673 to 1,210,329 _RS25120 BPHYT_RS25125: BPHYT_RS25125 - hypothetical protein, at 1,210,387 to 1,210,650 _RS25125 Position (kb) 1206 1207 1208 1209 1210Strain fitness (log2 ratio) -2 -1 0 1at 1205.914 kb on - strand, within BPHYT_RS25105at 1205.915 kb on + strand, within BPHYT_RS25105at 1206.572 kb on - strand, within BPHYT_RS25110at 1206.955 kb on - strandat 1207.056 kb on + strand, within BPHYT_RS25115at 1207.057 kb on - strand, within BPHYT_RS25115at 1207.060 kb on - strand, within BPHYT_RS25115at 1207.149 kb on - strand, within BPHYT_RS25115at 1207.617 kb on + strand, within BPHYT_RS25115at 1207.630 kb on - strand, within BPHYT_RS25115at 1207.670 kb on + strand, within BPHYT_RS25115at 1207.867 kb on - strand, within BPHYT_RS25115at 1208.325 kb on + strand, within BPHYT_RS25115at 1208.327 kb on + strand, within BPHYT_RS25115at 1208.449 kb on - strand, within BPHYT_RS25115at 1208.850 kb on + strand, within BPHYT_RS25115at 1209.133 kb on - strand, within BPHYT_RS25115at 1209.216 kb on + strand, within BPHYT_RS25115at 1209.392 kb on + strandat 1210.282 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10
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1,205,914 - BPHYT_RS25105 0.68 -0.1
1,205,915 + BPHYT_RS25105 0.68 +0.1
1,206,572 - BPHYT_RS25110 0.67 +0.2
1,206,955 - +0.3
1,207,056 + BPHYT_RS25115 0.12 -0.3
1,207,057 - BPHYT_RS25115 0.13 -2.5
1,207,060 - BPHYT_RS25115 0.13 +1.0
1,207,149 - BPHYT_RS25115 0.16 -0.0
1,207,617 + BPHYT_RS25115 0.31 -0.0
1,207,630 - BPHYT_RS25115 0.32 +0.2
1,207,670 + BPHYT_RS25115 0.33 +0.4
1,207,867 - BPHYT_RS25115 0.39 -1.0
1,208,325 + BPHYT_RS25115 0.55 +0.8
1,208,327 + BPHYT_RS25115 0.55 +0.6
1,208,449 - BPHYT_RS25115 0.59 +0.1
1,208,850 + BPHYT_RS25115 0.72 -0.8
1,209,133 - BPHYT_RS25115 0.82 +1.6
1,209,216 + BPHYT_RS25115 0.84 +1.2
1,209,392 + -0.4
1,210,282 + -0.4

Or see this region's nucleotide sequence