Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24245 and BPHYT_RS24250 are separated by 255 nucleotides BPHYT_RS24250 and BPHYT_RS24255 are separated by 198 nucleotides BPHYT_RS24255 and BPHYT_RS24260 are separated by 216 nucleotides
BPHYT_RS24245: BPHYT_RS24245 - MerR family transcriptional regulator, at 1,005,743 to 1,006,609
_RS24245
BPHYT_RS24250: BPHYT_RS24250 - GCN5 family acetyltransferase, at 1,006,865 to 1,007,458
_RS24250
BPHYT_RS24255: BPHYT_RS24255 - hypothetical protein, at 1,007,657 to 1,007,935
_RS24255
BPHYT_RS24260: BPHYT_RS24260 - RpiR family transcriptional regulator, at 1,008,152 to 1,009,048
_RS24260
Position (kb)
1006
1007
1008 Strain fitness (log2 ratio)
-1
0
1 at 1006.301 kb on - strand, within BPHYT_RS24245 at 1006.362 kb on + strand, within BPHYT_RS24245 at 1006.419 kb on + strand, within BPHYT_RS24245 at 1006.420 kb on - strand, within BPHYT_RS24245 at 1006.531 kb on - strand at 1007.247 kb on + strand, within BPHYT_RS24250 at 1007.247 kb on + strand, within BPHYT_RS24250 at 1007.288 kb on + strand, within BPHYT_RS24250 at 1007.353 kb on - strand, within BPHYT_RS24250 at 1007.458 kb on + strand at 1007.459 kb on - strand at 1007.459 kb on - strand at 1007.499 kb on - strand at 1007.501 kb on + strand at 1007.502 kb on - strand at 1007.551 kb on + strand at 1007.599 kb on - strand at 1007.658 kb on + strand at 1007.659 kb on - strand at 1007.659 kb on - strand at 1007.663 kb on - strand at 1007.663 kb on - strand at 1007.671 kb on - strand at 1007.675 kb on - strand at 1007.713 kb on - strand, within BPHYT_RS24255 at 1007.723 kb on - strand, within BPHYT_RS24255 at 1007.880 kb on + strand, within BPHYT_RS24255 at 1008.433 kb on - strand, within BPHYT_RS24260
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 1,006,301 - BPHYT_RS24245 0.64 +0.2 1,006,362 + BPHYT_RS24245 0.71 +0.2 1,006,419 + BPHYT_RS24245 0.78 -0.1 1,006,420 - BPHYT_RS24245 0.78 -0.2 1,006,531 - +0.1 1,007,247 + BPHYT_RS24250 0.64 +0.1 1,007,247 + BPHYT_RS24250 0.64 +1.0 1,007,288 + BPHYT_RS24250 0.71 -0.6 1,007,353 - BPHYT_RS24250 0.82 +0.8 1,007,458 + +0.1 1,007,459 - +1.4 1,007,459 - -0.9 1,007,499 - -0.3 1,007,501 + -0.5 1,007,502 - -0.1 1,007,551 + -1.4 1,007,599 - +0.1 1,007,658 + +0.4 1,007,659 - -0.3 1,007,659 - -1.2 1,007,663 - +0.0 1,007,663 - +0.4 1,007,671 - +0.7 1,007,675 - -0.0 1,007,713 - BPHYT_RS24255 0.20 -1.5 1,007,723 - BPHYT_RS24255 0.24 -0.1 1,007,880 + BPHYT_RS24255 0.80 +0.0 1,008,433 - BPHYT_RS24260 0.31 +0.2
Or see this region's nucleotide sequence