Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23730 and BPHYT_RS23735 are separated by 133 nucleotides BPHYT_RS23735 and BPHYT_RS23740 are separated by 185 nucleotides
BPHYT_RS23730: BPHYT_RS23730 - multidrug DMT transporter permease, at 891,286 to 892,158
_RS23730
BPHYT_RS23735: BPHYT_RS23735 - ABC transporter, at 892,292 to 893,851
_RS23735
BPHYT_RS23740: BPHYT_RS23740 - AraC family transcriptional regulator, at 894,037 to 894,792
_RS23740
Position (kb) 892
893
894 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 891.371 kb on + strand at 891.372 kb on - strand at 891.932 kb on + strand, within BPHYT_RS23730 at 891.932 kb on + strand, within BPHYT_RS23730 at 891.959 kb on - strand, within BPHYT_RS23730 at 892.421 kb on - strand at 892.483 kb on + strand, within BPHYT_RS23735 at 892.484 kb on - strand, within BPHYT_RS23735 at 892.484 kb on - strand, within BPHYT_RS23735 at 892.565 kb on + strand, within BPHYT_RS23735 at 892.579 kb on + strand, within BPHYT_RS23735 at 892.579 kb on + strand, within BPHYT_RS23735 at 892.579 kb on + strand, within BPHYT_RS23735 at 892.580 kb on - strand, within BPHYT_RS23735 at 892.588 kb on + strand, within BPHYT_RS23735 at 892.589 kb on - strand, within BPHYT_RS23735 at 892.851 kb on + strand, within BPHYT_RS23735 at 893.146 kb on - strand, within BPHYT_RS23735 at 893.722 kb on + strand at 893.829 kb on - strand at 893.934 kb on + strand at 893.934 kb on + strand at 893.934 kb on + strand at 893.934 kb on + strand at 893.935 kb on - strand at 893.969 kb on + strand at 893.970 kb on - strand at 893.973 kb on - strand at 893.973 kb on - strand at 893.973 kb on - strand at 894.035 kb on - strand at 894.035 kb on - strand at 894.035 kb on - strand at 894.061 kb on + strand at 894.061 kb on + strand at 894.062 kb on - strand at 894.062 kb on - strand at 894.062 kb on - strand at 894.238 kb on - strand, within BPHYT_RS23740 at 894.388 kb on + strand, within BPHYT_RS23740 at 894.389 kb on - strand, within BPHYT_RS23740 at 894.473 kb on + strand, within BPHYT_RS23740 at 894.474 kb on - strand, within BPHYT_RS23740 at 894.474 kb on - strand, within BPHYT_RS23740 at 894.474 kb on - strand, within BPHYT_RS23740 at 894.583 kb on + strand, within BPHYT_RS23740 at 894.835 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 891,371 + +1.6 891,372 - -0.2 891,932 + BPHYT_RS23730 0.74 +1.6 891,932 + BPHYT_RS23730 0.74 -1.2 891,959 - BPHYT_RS23730 0.77 -1.5 892,421 - +0.5 892,483 + BPHYT_RS23735 0.12 +0.4 892,484 - BPHYT_RS23735 0.12 -0.4 892,484 - BPHYT_RS23735 0.12 -0.0 892,565 + BPHYT_RS23735 0.17 +0.0 892,579 + BPHYT_RS23735 0.18 -1.7 892,579 + BPHYT_RS23735 0.18 -0.8 892,579 + BPHYT_RS23735 0.18 +0.1 892,580 - BPHYT_RS23735 0.18 +0.2 892,588 + BPHYT_RS23735 0.19 +0.2 892,589 - BPHYT_RS23735 0.19 +1.1 892,851 + BPHYT_RS23735 0.36 +0.1 893,146 - BPHYT_RS23735 0.55 +0.2 893,722 + +0.8 893,829 - +0.4 893,934 + -0.1 893,934 + +0.6 893,934 + +1.7 893,934 + +2.4 893,935 - +1.8 893,969 + +0.1 893,970 - -0.6 893,973 - -0.6 893,973 - -0.7 893,973 - -0.8 894,035 - +0.6 894,035 - -0.7 894,035 - -1.2 894,061 + -1.2 894,061 + -0.6 894,062 - -2.3 894,062 - -1.6 894,062 - -2.9 894,238 - BPHYT_RS23740 0.27 -0.6 894,388 + BPHYT_RS23740 0.46 -0.0 894,389 - BPHYT_RS23740 0.47 +0.6 894,473 + BPHYT_RS23740 0.58 -3.6 894,474 - BPHYT_RS23740 0.58 -0.8 894,474 - BPHYT_RS23740 0.58 -1.9 894,474 - BPHYT_RS23740 0.58 -2.2 894,583 + BPHYT_RS23740 0.72 -3.0 894,835 - +0.2
Or see this region's nucleotide sequence