Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22750

Experiment: 36hr PHYTO8 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22745 and BPHYT_RS22750 are separated by 446 nucleotidesBPHYT_RS22750 and BPHYT_RS22755 are separated by 250 nucleotides BPHYT_RS22745: BPHYT_RS22745 - sugar ABC transporter, at 659,938 to 660,942 _RS22745 BPHYT_RS22750: BPHYT_RS22750 - Flagellar brake protein YcgR 2, at 661,389 to 662,162 _RS22750 BPHYT_RS22755: BPHYT_RS22755 - LacI family transcription regulator, at 662,413 to 663,444 _RS22755 Position (kb) 661 662 663Strain fitness (log2 ratio) -2 -1 0 1at 660.488 kb on + strand, within BPHYT_RS22745at 660.872 kb on - strandat 661.274 kb on + strandat 661.275 kb on - strandat 661.306 kb on - strandat 661.726 kb on + strand, within BPHYT_RS22750at 661.778 kb on - strand, within BPHYT_RS22750at 662.169 kb on - strandat 662.169 kb on - strandat 662.256 kb on - strandat 662.256 kb on - strandat 662.403 kb on + strandat 662.514 kb on - strandat 662.540 kb on - strand, within BPHYT_RS22755at 662.540 kb on - strand, within BPHYT_RS22755

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10
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660,488 + BPHYT_RS22745 0.55 +1.0
660,872 - +0.6
661,274 + -0.2
661,275 - -2.3
661,306 - -0.6
661,726 + BPHYT_RS22750 0.44 -1.8
661,778 - BPHYT_RS22750 0.50 -1.5
662,169 - -0.2
662,169 - -0.4
662,256 - +0.7
662,256 - -0.4
662,403 + +0.6
662,514 - +0.8
662,540 - BPHYT_RS22755 0.12 +0.0
662,540 - BPHYT_RS22755 0.12 -0.2

Or see this region's nucleotide sequence