Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22505

Experiment: 36hr PHYTO8 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22500 and BPHYT_RS22505 are separated by 183 nucleotidesBPHYT_RS22505 and BPHYT_RS22510 are separated by 34 nucleotides BPHYT_RS22500: BPHYT_RS22500 - transcriptional regulator, at 606,467 to 607,471 _RS22500 BPHYT_RS22505: BPHYT_RS22505 - alpha/beta hydrolase, at 607,655 to 608,518 _RS22505 BPHYT_RS22510: BPHYT_RS22510 - MexE family multidrug efflux RND transporter periplasmic adaptor subunit, at 608,553 to 609,827 _RS22510 Position (kb) 607 608 609Strain fitness (log2 ratio) -2 -1 0 1 2at 606.655 kb on - strand, within BPHYT_RS22500at 606.897 kb on + strand, within BPHYT_RS22500at 606.898 kb on - strand, within BPHYT_RS22500at 607.787 kb on - strand, within BPHYT_RS22505at 607.884 kb on - strand, within BPHYT_RS22505at 608.255 kb on - strand, within BPHYT_RS22505at 608.361 kb on - strandat 608.517 kb on - strandat 608.517 kb on - strandat 608.558 kb on + strandat 609.211 kb on - strand, within BPHYT_RS22510at 609.240 kb on + strand, within BPHYT_RS22510at 609.241 kb on - strand, within BPHYT_RS22510at 609.430 kb on - strand, within BPHYT_RS22510at 609.430 kb on - strand, within BPHYT_RS22510

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10
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606,655 - BPHYT_RS22500 0.19 +0.3
606,897 + BPHYT_RS22500 0.43 +0.3
606,898 - BPHYT_RS22500 0.43 +0.9
607,787 - BPHYT_RS22505 0.15 +0.2
607,884 - BPHYT_RS22505 0.27 +0.0
608,255 - BPHYT_RS22505 0.69 +1.4
608,361 - +1.0
608,517 - +2.1
608,517 - +1.1
608,558 + +1.0
609,211 - BPHYT_RS22510 0.52 +1.0
609,240 + BPHYT_RS22510 0.54 -0.7
609,241 - BPHYT_RS22510 0.54 +0.3
609,430 - BPHYT_RS22510 0.69 -2.5
609,430 - BPHYT_RS22510 0.69 -0.2

Or see this region's nucleotide sequence