Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21610 and BPHYT_RS21615 are separated by 117 nucleotides BPHYT_RS21615 and BPHYT_RS21620 overlap by 4 nucleotides BPHYT_RS21620 and BPHYT_RS21625 are separated by 117 nucleotides
BPHYT_RS21610: BPHYT_RS21610 - DNA-binding protein, at 404,332 to 405,861
_RS21610
BPHYT_RS21615: BPHYT_RS21615 - FMN-dependent NADH-azoreductase, at 405,979 to 406,653
_RS21615
BPHYT_RS21620: BPHYT_RS21620 - GCN5 family acetyltransferase, at 406,650 to 407,117
_RS21620
BPHYT_RS21625: BPHYT_RS21625 - LysR family transcriptional regulator, at 407,235 to 408,149
_RS21625
Position (kb) 405
406
407 Strain fitness (log2 ratio) -1
0
1 at 405.349 kb on + strand, within BPHYT_RS21610 at 405.397 kb on + strand, within BPHYT_RS21610 at 405.548 kb on - strand, within BPHYT_RS21610 at 405.548 kb on - strand, within BPHYT_RS21610 at 405.548 kb on - strand, within BPHYT_RS21610 at 405.563 kb on + strand, within BPHYT_RS21610 at 405.872 kb on - strand at 405.872 kb on - strand at 405.997 kb on - strand at 405.997 kb on - strand at 406.184 kb on + strand, within BPHYT_RS21615 at 406.735 kb on + strand, within BPHYT_RS21620 at 407.320 kb on + strand at 407.321 kb on - strand at 407.623 kb on + strand, within BPHYT_RS21625
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 405,349 + BPHYT_RS21610 0.66 +0.0 405,397 + BPHYT_RS21610 0.70 -1.2 405,548 - BPHYT_RS21610 0.79 -1.6 405,548 - BPHYT_RS21610 0.79 -0.1 405,548 - BPHYT_RS21610 0.79 +0.5 405,563 + BPHYT_RS21610 0.80 -0.1 405,872 - +1.1 405,872 - +0.1 405,997 - -1.3 405,997 - -0.6 406,184 + BPHYT_RS21615 0.30 -0.9 406,735 + BPHYT_RS21620 0.18 -1.2 407,320 + -0.6 407,321 - +0.5 407,623 + BPHYT_RS21625 0.42 +0.3
Or see this region's nucleotide sequence