Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18910 and BPHYT_RS18915 are separated by 308 nucleotides BPHYT_RS18915 and BPHYT_RS18920 overlap by 4 nucleotides BPHYT_RS18920 and BPHYT_RS18925 are separated by 60 nucleotides BPHYT_RS18925 and BPHYT_RS18930 are separated by 28 nucleotides
BPHYT_RS18910: BPHYT_RS18910 - FlgN, at 4,248,191 to 4,248,637
_RS18910
BPHYT_RS18915: BPHYT_RS18915 - hypothetical protein, at 4,248,946 to 4,249,128
_RS18915
BPHYT_RS18920: BPHYT_RS18920 - hypothetical protein, at 4,249,125 to 4,249,535
_RS18920
BPHYT_RS18925: BPHYT_RS18925 - RNA polymerase sigma70, at 4,249,596 to 4,250,330
_RS18925
BPHYT_RS18930: BPHYT_RS18930 - flagellar biosynthesis protein FlhG, at 4,250,359 to 4,251,228
_RS18930
Position (kb)
4249
4250 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4248.503 kb on - strand, within BPHYT_RS18910 at 4248.539 kb on + strand, within BPHYT_RS18910 at 4248.540 kb on - strand, within BPHYT_RS18910 at 4248.596 kb on + strand at 4248.597 kb on - strand at 4248.775 kb on - strand at 4248.837 kb on + strand at 4248.837 kb on + strand at 4248.838 kb on - strand at 4248.896 kb on + strand at 4248.945 kb on + strand at 4249.411 kb on + strand, within BPHYT_RS18920 at 4249.411 kb on + strand, within BPHYT_RS18920 at 4249.415 kb on + strand, within BPHYT_RS18920 at 4249.415 kb on + strand, within BPHYT_RS18920 at 4249.421 kb on + strand, within BPHYT_RS18920 at 4249.421 kb on + strand, within BPHYT_RS18920 at 4249.461 kb on + strand, within BPHYT_RS18920 at 4249.738 kb on + strand, within BPHYT_RS18925 at 4249.738 kb on + strand, within BPHYT_RS18925 at 4249.739 kb on - strand, within BPHYT_RS18925 at 4249.903 kb on + strand, within BPHYT_RS18925 at 4249.906 kb on + strand, within BPHYT_RS18925 at 4249.907 kb on - strand, within BPHYT_RS18925 at 4249.907 kb on - strand, within BPHYT_RS18925 at 4249.948 kb on + strand, within BPHYT_RS18925 at 4249.949 kb on - strand, within BPHYT_RS18925 at 4249.949 kb on - strand, within BPHYT_RS18925 at 4250.131 kb on + strand, within BPHYT_RS18925 at 4250.165 kb on - strand, within BPHYT_RS18925 at 4250.281 kb on + strand at 4250.282 kb on - strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.327 kb on - strand at 4250.327 kb on - strand at 4250.327 kb on - strand at 4250.327 kb on - strand at 4250.463 kb on - strand, within BPHYT_RS18930 at 4250.463 kb on - strand, within BPHYT_RS18930
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 4,248,503 - BPHYT_RS18910 0.70 +0.4 4,248,539 + BPHYT_RS18910 0.78 +1.3 4,248,540 - BPHYT_RS18910 0.78 +1.0 4,248,596 + -1.4 4,248,597 - -0.0 4,248,775 - -0.8 4,248,837 + -0.2 4,248,837 + +0.7 4,248,838 - -0.2 4,248,896 + +0.1 4,248,945 + +1.1 4,249,411 + BPHYT_RS18920 0.70 +0.0 4,249,411 + BPHYT_RS18920 0.70 +3.3 4,249,415 + BPHYT_RS18920 0.71 -0.4 4,249,415 + BPHYT_RS18920 0.71 +0.1 4,249,421 + BPHYT_RS18920 0.72 +0.6 4,249,421 + BPHYT_RS18920 0.72 +0.2 4,249,461 + BPHYT_RS18920 0.82 +0.4 4,249,738 + BPHYT_RS18925 0.19 -0.0 4,249,738 + BPHYT_RS18925 0.19 +0.5 4,249,739 - BPHYT_RS18925 0.19 -0.3 4,249,903 + BPHYT_RS18925 0.42 -0.0 4,249,906 + BPHYT_RS18925 0.42 -0.2 4,249,907 - BPHYT_RS18925 0.42 -0.4 4,249,907 - BPHYT_RS18925 0.42 +0.3 4,249,948 + BPHYT_RS18925 0.48 -0.9 4,249,949 - BPHYT_RS18925 0.48 -0.8 4,249,949 - BPHYT_RS18925 0.48 -0.8 4,250,131 + BPHYT_RS18925 0.73 +0.1 4,250,165 - BPHYT_RS18925 0.77 -0.5 4,250,281 + +1.6 4,250,282 - -0.5 4,250,326 + -1.3 4,250,326 + +0.1 4,250,326 + +0.9 4,250,326 + -0.0 4,250,326 + -0.6 4,250,327 - -0.0 4,250,327 - -1.1 4,250,327 - -1.0 4,250,327 - -0.2 4,250,463 - BPHYT_RS18930 0.12 -1.8 4,250,463 - BPHYT_RS18930 0.12 -0.2
Or see this region's nucleotide sequence