Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09225 and BPHYT_RS09230 are separated by 177 nucleotides BPHYT_RS09230 and BPHYT_RS09235 are separated by 353 nucleotides BPHYT_RS09235 and BPHYT_RS09240 are separated by 38 nucleotides
BPHYT_RS09225: BPHYT_RS09225 - LysR family transcriptional regulator, at 2,088,891 to 2,089,832
_RS09225
BPHYT_RS09230: BPHYT_RS09230 - NADPH:quinone reductase, at 2,090,010 to 2,091,032
_RS09230
BPHYT_RS09235: BPHYT_RS09235 - hypothetical protein, at 2,091,386 to 2,091,943
_RS09235
BPHYT_RS09240: BPHYT_RS09240 - ABC transporter permease, at 2,091,982 to 2,092,788
_RS09240
Position (kb)
2090
2091
2092 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2089.028 kb on - strand, within BPHYT_RS09225 at 2089.066 kb on + strand, within BPHYT_RS09225 at 2089.066 kb on + strand, within BPHYT_RS09225 at 2089.067 kb on - strand, within BPHYT_RS09225 at 2089.516 kb on - strand at 2089.741 kb on - strand at 2089.911 kb on + strand at 2089.911 kb on + strand at 2090.010 kb on - strand at 2090.010 kb on - strand at 2090.034 kb on + strand at 2090.035 kb on - strand at 2090.216 kb on + strand, within BPHYT_RS09230 at 2090.275 kb on - strand, within BPHYT_RS09230 at 2090.275 kb on - strand, within BPHYT_RS09230 at 2091.127 kb on + strand at 2091.142 kb on + strand at 2091.285 kb on - strand at 2091.342 kb on - strand at 2091.386 kb on - strand at 2091.386 kb on - strand at 2091.526 kb on + strand, within BPHYT_RS09235 at 2091.526 kb on + strand, within BPHYT_RS09235 at 2091.526 kb on + strand, within BPHYT_RS09235 at 2091.662 kb on + strand, within BPHYT_RS09235 at 2091.663 kb on - strand, within BPHYT_RS09235
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 2,089,028 - BPHYT_RS09225 0.15 +1.0 2,089,066 + BPHYT_RS09225 0.19 +1.5 2,089,066 + BPHYT_RS09225 0.19 -1.9 2,089,067 - BPHYT_RS09225 0.19 -1.1 2,089,516 - +2.5 2,089,741 - -0.5 2,089,911 + +0.3 2,089,911 + -1.3 2,090,010 - +0.1 2,090,010 - -0.0 2,090,034 + +0.8 2,090,035 - -1.5 2,090,216 + BPHYT_RS09230 0.20 -1.7 2,090,275 - BPHYT_RS09230 0.26 -0.2 2,090,275 - BPHYT_RS09230 0.26 -1.5 2,091,127 + +0.4 2,091,142 + -0.9 2,091,285 - +0.4 2,091,342 - +0.9 2,091,386 - -0.6 2,091,386 - +0.1 2,091,526 + BPHYT_RS09235 0.25 +0.1 2,091,526 + BPHYT_RS09235 0.25 +1.1 2,091,526 + BPHYT_RS09235 0.25 +1.4 2,091,662 + BPHYT_RS09235 0.49 -1.8 2,091,663 - BPHYT_RS09235 0.50 -0.6
Or see this region's nucleotide sequence