Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08635 and BPHYT_RS08640 are separated by 504 nucleotides BPHYT_RS08640 and BPHYT_RS08645 are separated by 51 nucleotides BPHYT_RS08645 and BPHYT_RS08650 overlap by 4 nucleotides
BPHYT_RS08635: BPHYT_RS08635 - membrane protein, at 1,968,293 to 1,968,718
_RS08635
BPHYT_RS08640: BPHYT_RS08640 - alkanesulfonate monooxygenase, at 1,969,223 to 1,970,383
_RS08640
BPHYT_RS08645: BPHYT_RS08645 - sulfonate ABC transporter, at 1,970,435 to 1,971,277
_RS08645
BPHYT_RS08650: BPHYT_RS08650 - sulfonate ABC transporter ATP-binding protein, at 1,971,274 to 1,972,275
_RS08650
Position (kb)
1969
1970
1971 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1968.771 kb on + strand at 1968.792 kb on + strand at 1968.793 kb on - strand at 1968.793 kb on - strand at 1968.793 kb on - strand at 1968.793 kb on - strand at 1968.793 kb on - strand at 1968.794 kb on + strand at 1968.794 kb on + strand at 1968.795 kb on - strand at 1968.795 kb on - strand at 1968.795 kb on - strand at 1968.796 kb on + strand at 1968.796 kb on + strand at 1968.796 kb on + strand at 1968.796 kb on + strand at 1968.797 kb on - strand at 1968.797 kb on - strand at 1968.797 kb on - strand at 1968.798 kb on + strand at 1968.799 kb on - strand at 1968.805 kb on - strand at 1968.805 kb on - strand at 1968.847 kb on + strand at 1968.879 kb on + strand at 1969.192 kb on + strand at 1969.347 kb on - strand, within BPHYT_RS08640 at 1969.719 kb on - strand, within BPHYT_RS08640 at 1969.798 kb on + strand, within BPHYT_RS08640 at 1969.798 kb on + strand, within BPHYT_RS08640 at 1970.253 kb on - strand, within BPHYT_RS08640 at 1970.354 kb on + strand at 1970.355 kb on - strand at 1970.355 kb on - strand at 1970.906 kb on + strand, within BPHYT_RS08645 at 1971.255 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 1,968,771 + +1.5 1,968,792 + -0.1 1,968,793 - +1.2 1,968,793 - -0.8 1,968,793 - -1.2 1,968,793 - -0.9 1,968,793 - -0.1 1,968,794 + +0.4 1,968,794 + +0.6 1,968,795 - -0.6 1,968,795 - +0.4 1,968,795 - -0.5 1,968,796 + -1.2 1,968,796 + +0.5 1,968,796 + +0.9 1,968,796 + -0.3 1,968,797 - -1.8 1,968,797 - -0.5 1,968,797 - -0.1 1,968,798 + -0.6 1,968,799 - -0.2 1,968,805 - -1.3 1,968,805 - -1.6 1,968,847 + -0.1 1,968,879 + -1.2 1,969,192 + -0.6 1,969,347 - BPHYT_RS08640 0.11 +0.1 1,969,719 - BPHYT_RS08640 0.43 -0.7 1,969,798 + BPHYT_RS08640 0.50 -0.2 1,969,798 + BPHYT_RS08640 0.50 +0.1 1,970,253 - BPHYT_RS08640 0.89 +1.8 1,970,354 + +0.4 1,970,355 - -0.1 1,970,355 - -0.1 1,970,906 + BPHYT_RS08645 0.56 -1.2 1,971,255 - -0.7
Or see this region's nucleotide sequence