Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05395 and BPHYT_RS05400 are separated by 344 nucleotides BPHYT_RS05400 and BPHYT_RS05405 are separated by 33 nucleotides BPHYT_RS05405 and BPHYT_RS05410 are separated by 144 nucleotides
BPHYT_RS05395: BPHYT_RS05395 - hypothetical protein, at 1,240,439 to 1,240,627
_RS05395
BPHYT_RS05400: BPHYT_RS05400 - hypothetical protein, at 1,240,972 to 1,241,418
_RS05400
BPHYT_RS05405: BPHYT_RS05405 - GCN5 family acetyltransferase, at 1,241,452 to 1,241,955
_RS05405
BPHYT_RS05410: BPHYT_RS05410 - beta-lactamase, at 1,242,100 to 1,243,104
_RS05410
Position (kb)
1241
1242 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1240.517 kb on - strand, within BPHYT_RS05395 at 1241.499 kb on - strand at 1241.670 kb on - strand, within BPHYT_RS05405 at 1241.866 kb on + strand, within BPHYT_RS05405 at 1241.872 kb on - strand, within BPHYT_RS05405 at 1241.994 kb on + strand at 1241.995 kb on - strand at 1241.997 kb on - strand at 1242.067 kb on + strand at 1242.069 kb on + strand at 1242.161 kb on - strand at 1242.632 kb on - strand, within BPHYT_RS05410 at 1242.644 kb on + strand, within BPHYT_RS05410 at 1242.690 kb on + strand, within BPHYT_RS05410 at 1242.691 kb on - strand, within BPHYT_RS05410 at 1242.791 kb on + strand, within BPHYT_RS05410 at 1242.929 kb on - strand, within BPHYT_RS05410
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 1,240,517 - BPHYT_RS05395 0.41 +0.8 1,241,499 - +0.2 1,241,670 - BPHYT_RS05405 0.43 -2.3 1,241,866 + BPHYT_RS05405 0.82 -1.7 1,241,872 - BPHYT_RS05405 0.83 -1.1 1,241,994 + -0.3 1,241,995 - -1.5 1,241,997 - +3.6 1,242,067 + -1.2 1,242,069 + -0.2 1,242,161 - -0.6 1,242,632 - BPHYT_RS05410 0.53 -0.6 1,242,644 + BPHYT_RS05410 0.54 +0.7 1,242,690 + BPHYT_RS05410 0.59 -0.3 1,242,691 - BPHYT_RS05410 0.59 -1.7 1,242,791 + BPHYT_RS05410 0.69 -0.8 1,242,929 - BPHYT_RS05410 0.82 -0.0
Or see this region's nucleotide sequence