Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04325 and BPHYT_RS04330 are separated by 125 nucleotides BPHYT_RS04330 and BPHYT_RS04335 are separated by 53 nucleotides BPHYT_RS04335 and BPHYT_RS04340 overlap by 4 nucleotides
BPHYT_RS04325: BPHYT_RS04325 - acyltransferase 3, at 995,925 to 997,088
_RS04325
BPHYT_RS04330: BPHYT_RS04330 - hypothetical protein, at 997,214 to 998,356
_RS04330
BPHYT_RS04335: BPHYT_RS04335 - membrane protein, at 998,410 to 998,835
_RS04335
BPHYT_RS04340: BPHYT_RS04340 - hypothetical protein, at 998,832 to 1,000,115
_RS04340
Position (kb) 997
998
999 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 996.301 kb on - strand, within BPHYT_RS04325 at 996.363 kb on + strand, within BPHYT_RS04325 at 996.487 kb on + strand, within BPHYT_RS04325 at 996.509 kb on + strand, within BPHYT_RS04325 at 996.540 kb on - strand, within BPHYT_RS04325 at 996.705 kb on + strand, within BPHYT_RS04325 at 996.716 kb on + strand, within BPHYT_RS04325 at 996.873 kb on + strand, within BPHYT_RS04325 at 997.086 kb on + strand at 997.113 kb on + strand at 997.114 kb on - strand at 997.203 kb on + strand at 997.204 kb on - strand at 997.204 kb on - strand at 997.207 kb on + strand at 997.239 kb on - strand at 997.352 kb on + strand, within BPHYT_RS04330 at 997.352 kb on + strand, within BPHYT_RS04330 at 997.353 kb on - strand, within BPHYT_RS04330 at 997.353 kb on - strand, within BPHYT_RS04330 at 997.360 kb on + strand, within BPHYT_RS04330 at 997.360 kb on + strand, within BPHYT_RS04330 at 997.360 kb on + strand, within BPHYT_RS04330 at 997.446 kb on - strand, within BPHYT_RS04330 at 997.576 kb on + strand, within BPHYT_RS04330 at 997.576 kb on + strand, within BPHYT_RS04330 at 997.576 kb on + strand, within BPHYT_RS04330 at 998.117 kb on + strand, within BPHYT_RS04330 at 998.203 kb on + strand, within BPHYT_RS04330 at 998.203 kb on + strand, within BPHYT_RS04330 at 998.204 kb on - strand, within BPHYT_RS04330 at 998.215 kb on - strand, within BPHYT_RS04330 at 998.215 kb on - strand, within BPHYT_RS04330 at 998.528 kb on + strand, within BPHYT_RS04335 at 998.528 kb on + strand, within BPHYT_RS04335 at 998.529 kb on - strand, within BPHYT_RS04335 at 998.836 kb on - strand at 998.836 kb on - strand at 998.899 kb on + strand at 998.900 kb on - strand at 998.900 kb on - strand at 998.902 kb on + strand at 998.965 kb on + strand, within BPHYT_RS04340 at 999.212 kb on + strand, within BPHYT_RS04340 at 999.343 kb on + strand, within BPHYT_RS04340
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 996,301 - BPHYT_RS04325 0.32 +1.0 996,363 + BPHYT_RS04325 0.38 -1.2 996,487 + BPHYT_RS04325 0.48 -1.5 996,509 + BPHYT_RS04325 0.50 -2.5 996,540 - BPHYT_RS04325 0.53 -2.1 996,705 + BPHYT_RS04325 0.67 -0.9 996,716 + BPHYT_RS04325 0.68 +0.2 996,873 + BPHYT_RS04325 0.81 -1.0 997,086 + +0.6 997,113 + -0.2 997,114 - -0.6 997,203 + -2.9 997,204 - -1.2 997,204 - -3.8 997,207 + -1.4 997,239 - -1.5 997,352 + BPHYT_RS04330 0.12 +0.3 997,352 + BPHYT_RS04330 0.12 -2.1 997,353 - BPHYT_RS04330 0.12 -2.0 997,353 - BPHYT_RS04330 0.12 -1.7 997,360 + BPHYT_RS04330 0.13 -0.1 997,360 + BPHYT_RS04330 0.13 -2.8 997,360 + BPHYT_RS04330 0.13 +0.3 997,446 - BPHYT_RS04330 0.20 -2.6 997,576 + BPHYT_RS04330 0.32 -1.3 997,576 + BPHYT_RS04330 0.32 +0.7 997,576 + BPHYT_RS04330 0.32 -0.5 998,117 + BPHYT_RS04330 0.79 -1.5 998,203 + BPHYT_RS04330 0.87 -0.3 998,203 + BPHYT_RS04330 0.87 -2.0 998,204 - BPHYT_RS04330 0.87 -2.2 998,215 - BPHYT_RS04330 0.88 +0.5 998,215 - BPHYT_RS04330 0.88 +0.9 998,528 + BPHYT_RS04335 0.28 +0.6 998,528 + BPHYT_RS04335 0.28 -0.0 998,529 - BPHYT_RS04335 0.28 +2.6 998,836 - -1.8 998,836 - -2.7 998,899 + +1.0 998,900 - -0.2 998,900 - +4.2 998,902 + -0.6 998,965 + BPHYT_RS04340 0.10 +1.6 999,212 + BPHYT_RS04340 0.30 +0.1 999,343 + BPHYT_RS04340 0.40 +1.6
Or see this region's nucleotide sequence