Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02085 and BPHYT_RS02090 are separated by 224 nucleotides BPHYT_RS02090 and BPHYT_RS02095 are separated by 283 nucleotides
BPHYT_RS02085: BPHYT_RS02085 - methylmalonyl-CoA mutase, at 477,797 to 481,693
_RS02085
BPHYT_RS02090: BPHYT_RS02090 - 2-nitropropane dioxygenase, at 481,918 to 482,877
_RS02090
BPHYT_RS02095: BPHYT_RS02095 - alpha/beta hydrolase, at 483,161 to 484,051
_RS02095
Position (kb) 481
482
483 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 480.995 kb on + strand, within BPHYT_RS02085 at 480.996 kb on - strand, within BPHYT_RS02085 at 481.373 kb on + strand at 481.373 kb on + strand at 481.373 kb on + strand at 481.373 kb on + strand at 481.374 kb on - strand at 481.374 kb on - strand at 481.374 kb on - strand at 481.420 kb on + strand at 481.420 kb on + strand at 481.687 kb on + strand at 481.688 kb on - strand at 481.691 kb on + strand at 481.691 kb on + strand at 481.692 kb on - strand at 481.692 kb on - strand at 481.953 kb on + strand at 481.954 kb on - strand at 481.954 kb on - strand at 481.955 kb on + strand at 482.144 kb on + strand, within BPHYT_RS02090 at 482.144 kb on + strand, within BPHYT_RS02090 at 482.145 kb on - strand, within BPHYT_RS02090 at 482.145 kb on - strand, within BPHYT_RS02090 at 482.145 kb on - strand, within BPHYT_RS02090 at 482.175 kb on + strand, within BPHYT_RS02090 at 482.178 kb on - strand, within BPHYT_RS02090 at 482.220 kb on - strand, within BPHYT_RS02090 at 482.270 kb on + strand, within BPHYT_RS02090 at 482.270 kb on + strand, within BPHYT_RS02090 at 482.271 kb on - strand, within BPHYT_RS02090 at 482.271 kb on - strand, within BPHYT_RS02090 at 482.271 kb on - strand, within BPHYT_RS02090 at 482.357 kb on - strand, within BPHYT_RS02090 at 482.357 kb on - strand, within BPHYT_RS02090 at 482.510 kb on + strand, within BPHYT_RS02090 at 482.510 kb on + strand, within BPHYT_RS02090 at 482.511 kb on - strand, within BPHYT_RS02090 at 482.514 kb on + strand, within BPHYT_RS02090 at 482.515 kb on - strand, within BPHYT_RS02090 at 482.877 kb on + strand at 482.877 kb on + strand at 482.877 kb on + strand at 483.078 kb on + strand at 483.079 kb on - strand at 483.079 kb on - strand at 483.079 kb on - strand at 483.348 kb on - strand, within BPHYT_RS02095 at 483.570 kb on - strand, within BPHYT_RS02095 at 483.570 kb on - strand, within BPHYT_RS02095 at 483.570 kb on - strand, within BPHYT_RS02095 at 483.674 kb on + strand, within BPHYT_RS02095 at 483.675 kb on - strand, within BPHYT_RS02095 at 483.675 kb on - strand, within BPHYT_RS02095 at 483.675 kb on - strand, within BPHYT_RS02095 at 483.675 kb on - strand at 483.794 kb on + strand, within BPHYT_RS02095
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 480,995 + BPHYT_RS02085 0.82 +1.0 480,996 - BPHYT_RS02085 0.82 -0.3 481,373 + +0.6 481,373 + -0.1 481,373 + +2.6 481,373 + -0.2 481,374 - -0.9 481,374 - -0.6 481,374 - +0.8 481,420 + -0.3 481,420 + +0.8 481,687 + -1.6 481,688 - -0.4 481,691 + -0.2 481,691 + -0.9 481,692 - -0.3 481,692 - +0.2 481,953 + -0.4 481,954 - -2.2 481,954 - +0.9 481,955 + -0.0 482,144 + BPHYT_RS02090 0.24 +0.0 482,144 + BPHYT_RS02090 0.24 -0.6 482,145 - BPHYT_RS02090 0.24 -0.4 482,145 - BPHYT_RS02090 0.24 -0.8 482,145 - BPHYT_RS02090 0.24 -0.9 482,175 + BPHYT_RS02090 0.27 -0.5 482,178 - BPHYT_RS02090 0.27 -1.0 482,220 - BPHYT_RS02090 0.31 -2.2 482,270 + BPHYT_RS02090 0.37 -0.5 482,270 + BPHYT_RS02090 0.37 -0.4 482,271 - BPHYT_RS02090 0.37 +1.1 482,271 - BPHYT_RS02090 0.37 +0.9 482,271 - BPHYT_RS02090 0.37 -0.0 482,357 - BPHYT_RS02090 0.46 +0.1 482,357 - BPHYT_RS02090 0.46 -0.5 482,510 + BPHYT_RS02090 0.62 +1.5 482,510 + BPHYT_RS02090 0.62 +0.3 482,511 - BPHYT_RS02090 0.62 -1.6 482,514 + BPHYT_RS02090 0.62 -1.0 482,515 - BPHYT_RS02090 0.62 +1.6 482,877 + -0.2 482,877 + -0.8 482,877 + -0.4 483,078 + +0.3 483,079 - -0.0 483,079 - -1.8 483,079 - -0.8 483,348 - BPHYT_RS02095 0.21 -0.9 483,570 - BPHYT_RS02095 0.46 -2.0 483,570 - BPHYT_RS02095 0.46 +0.8 483,570 - BPHYT_RS02095 0.46 +1.3 483,674 + BPHYT_RS02095 0.58 -1.5 483,675 - BPHYT_RS02095 0.58 -1.6 483,675 - BPHYT_RS02095 0.58 -0.0 483,675 - BPHYT_RS02095 0.58 +0.3 483,675 - +3.6 483,794 + BPHYT_RS02095 0.71 -0.7
Or see this region's nucleotide sequence