Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01945 and BPHYT_RS01950 are separated by 216 nucleotides BPHYT_RS01950 and BPHYT_RS01955 are separated by 26 nucleotides BPHYT_RS01955 and BPHYT_RS01960 overlap by 1 nucleotides BPHYT_RS01960 and BPHYT_RS01965 are separated by 77 nucleotides BPHYT_RS01965 and BPHYT_RS01970 are separated by 149 nucleotides
BPHYT_RS01945: BPHYT_RS01945 - hypothetical protein, at 441,700 to 442,122
_RS01945
BPHYT_RS01950: BPHYT_RS01950 - phosphoheptose isomerase, at 442,339 to 442,926
_RS01950
BPHYT_RS01955: BPHYT_RS01955 - hypothetical protein, at 442,953 to 443,750
_RS01955
BPHYT_RS01960: BPHYT_RS01960 - cytochrome C, at 443,750 to 444,097
_RS01960
BPHYT_RS01965: BPHYT_RS01965 - tRNA-Ala, at 444,175 to 444,250
_RS01965
BPHYT_RS01970: BPHYT_RS01970 - methyl-accepting chemotaxis protein, at 444,400 to 446,157
_RS01970
Position (kb) 442
443
444 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 441.981 kb on + strand, within BPHYT_RS01945 at 441.981 kb on + strand, within BPHYT_RS01945 at 441.982 kb on - strand, within BPHYT_RS01945 at 441.982 kb on - strand, within BPHYT_RS01945 at 442.213 kb on + strand at 442.213 kb on + strand at 442.213 kb on + strand at 442.213 kb on + strand at 442.213 kb on + strand at 442.214 kb on - strand at 442.329 kb on + strand at 442.619 kb on - strand, within BPHYT_RS01950 at 442.799 kb on + strand, within BPHYT_RS01950 at 442.800 kb on - strand, within BPHYT_RS01950 at 442.800 kb on - strand, within BPHYT_RS01950 at 442.852 kb on - strand, within BPHYT_RS01950 at 442.947 kb on + strand at 442.947 kb on + strand at 442.948 kb on - strand at 442.948 kb on - strand at 442.961 kb on - strand at 442.961 kb on - strand at 443.356 kb on - strand, within BPHYT_RS01955 at 443.446 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955 at 443.936 kb on + strand, within BPHYT_RS01960 at 443.936 kb on + strand, within BPHYT_RS01960 at 443.936 kb on + strand, within BPHYT_RS01960 at 443.937 kb on - strand, within BPHYT_RS01960 at 444.156 kb on + strand at 444.182 kb on + strand at 444.182 kb on + strand at 444.183 kb on - strand, within BPHYT_RS01965 at 444.281 kb on - strand at 444.321 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 441,981 + BPHYT_RS01945 0.66 +0.6 441,981 + BPHYT_RS01945 0.66 +3.2 441,982 - BPHYT_RS01945 0.67 -1.1 441,982 - BPHYT_RS01945 0.67 +0.6 442,213 + +0.0 442,213 + +0.5 442,213 + +3.6 442,213 + -0.1 442,213 + -0.4 442,214 - -0.8 442,329 + +1.4 442,619 - BPHYT_RS01950 0.48 -1.3 442,799 + BPHYT_RS01950 0.78 +0.1 442,800 - BPHYT_RS01950 0.78 -0.6 442,800 - BPHYT_RS01950 0.78 -0.8 442,852 - BPHYT_RS01950 0.87 -2.2 442,947 + -2.9 442,947 + +1.3 442,948 - -1.8 442,948 - -0.8 442,961 - -1.9 442,961 - +0.6 443,356 - BPHYT_RS01955 0.51 -0.9 443,446 - BPHYT_RS01955 0.62 -0.3 443,542 - BPHYT_RS01955 0.74 -0.3 443,542 - BPHYT_RS01955 0.74 -1.6 443,542 - BPHYT_RS01955 0.74 -0.8 443,936 + BPHYT_RS01960 0.53 +1.0 443,936 + BPHYT_RS01960 0.53 +0.8 443,936 + BPHYT_RS01960 0.53 +1.5 443,937 - BPHYT_RS01960 0.54 +0.4 444,156 + -1.4 444,182 + -0.5 444,182 + -0.1 444,183 - BPHYT_RS01965 0.11 +0.3 444,281 - -1.3 444,321 + +0.6
Or see this region's nucleotide sequence