Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01230 and BPHYT_RS01235 are separated by 461 nucleotides BPHYT_RS01235 and BPHYT_RS01240 are separated by 196 nucleotides BPHYT_RS01240 and BPHYT_RS01245 are separated by 95 nucleotides BPHYT_RS01245 and BPHYT_RS01250 are separated by 54 nucleotides
BPHYT_RS01230: BPHYT_RS01230 - DNA-binding protein, at 284,011 to 284,307
_RS01230
BPHYT_RS01235: BPHYT_RS01235 - hypothetical protein, at 284,769 to 285,008
_RS01235
BPHYT_RS01240: BPHYT_RS01240 - hypothetical protein, at 285,205 to 285,423
_RS01240
BPHYT_RS01245: BPHYT_RS01245 - hypothetical protein, at 285,519 to 286,262
_RS01245
BPHYT_RS01250: BPHYT_RS01250 - integrase, at 286,317 to 287,315
_RS01250
Position (kb) 285
286 Strain fitness (log2 ratio) -1
0
1 at 284.227 kb on - strand, within BPHYT_RS01230 at 284.368 kb on + strand at 284.369 kb on - strand at 284.434 kb on + strand at 284.808 kb on + strand, within BPHYT_RS01235 at 284.809 kb on - strand, within BPHYT_RS01235 at 285.304 kb on - strand, within BPHYT_RS01240 at 285.381 kb on - strand, within BPHYT_RS01240 at 285.427 kb on - strand at 285.527 kb on + strand at 285.591 kb on + strand at 285.693 kb on + strand, within BPHYT_RS01245 at 285.694 kb on - strand, within BPHYT_RS01245 at 285.951 kb on + strand, within BPHYT_RS01245 at 285.951 kb on + strand, within BPHYT_RS01245 at 285.952 kb on - strand, within BPHYT_RS01245 at 285.952 kb on - strand, within BPHYT_RS01245 at 286.058 kb on - strand, within BPHYT_RS01245 at 286.058 kb on - strand, within BPHYT_RS01245 at 286.171 kb on + strand, within BPHYT_RS01245 at 286.172 kb on - strand, within BPHYT_RS01245 at 286.260 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 284,227 - BPHYT_RS01230 0.73 -0.9 284,368 + +0.1 284,369 - -0.4 284,434 + +1.2 284,808 + BPHYT_RS01235 0.16 -1.1 284,809 - BPHYT_RS01235 0.17 -1.3 285,304 - BPHYT_RS01240 0.45 +0.3 285,381 - BPHYT_RS01240 0.80 +0.8 285,427 - -0.7 285,527 + +0.9 285,591 + -0.5 285,693 + BPHYT_RS01245 0.23 +0.7 285,694 - BPHYT_RS01245 0.24 +1.2 285,951 + BPHYT_RS01245 0.58 -0.8 285,951 + BPHYT_RS01245 0.58 +0.2 285,952 - BPHYT_RS01245 0.58 -0.3 285,952 - BPHYT_RS01245 0.58 +1.1 286,058 - BPHYT_RS01245 0.72 -0.3 286,058 - BPHYT_RS01245 0.72 -0.9 286,171 + BPHYT_RS01245 0.88 +0.3 286,172 - BPHYT_RS01245 0.88 +0.1 286,260 + +0.2
Or see this region's nucleotide sequence