Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01190 and BPHYT_RS01195 are separated by 800 nucleotides BPHYT_RS01195 and BPHYT_RS01200 are separated by 52 nucleotides
BPHYT_RS01190: BPHYT_RS01190 - hypothetical protein, at 273,598 to 274,338
_RS01190
BPHYT_RS01195: BPHYT_RS01195 - hypothetical protein, at 275,139 to 277,109
_RS01195
BPHYT_RS01200: BPHYT_RS01200 - hypothetical protein, at 277,162 to 279,012
_RS01200
Position (kb) 275
276
277
278 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 274.376 kb on - strand at 274.573 kb on + strand at 274.659 kb on - strand at 274.671 kb on - strand at 274.672 kb on + strand at 274.702 kb on - strand at 274.835 kb on + strand at 274.836 kb on - strand at 274.887 kb on - strand at 274.943 kb on + strand at 275.272 kb on - strand at 275.276 kb on + strand at 275.277 kb on - strand at 275.277 kb on - strand at 275.304 kb on + strand at 275.411 kb on + strand, within BPHYT_RS01195 at 275.411 kb on + strand, within BPHYT_RS01195 at 275.412 kb on - strand, within BPHYT_RS01195 at 275.463 kb on + strand, within BPHYT_RS01195 at 275.623 kb on - strand, within BPHYT_RS01195 at 275.886 kb on - strand, within BPHYT_RS01195 at 275.928 kb on + strand, within BPHYT_RS01195 at 276.005 kb on - strand, within BPHYT_RS01195 at 276.005 kb on - strand, within BPHYT_RS01195 at 276.005 kb on - strand, within BPHYT_RS01195 at 276.005 kb on - strand, within BPHYT_RS01195 at 276.008 kb on + strand, within BPHYT_RS01195 at 276.122 kb on + strand, within BPHYT_RS01195 at 276.211 kb on - strand, within BPHYT_RS01195 at 276.440 kb on + strand, within BPHYT_RS01195 at 276.567 kb on + strand, within BPHYT_RS01195 at 276.576 kb on + strand, within BPHYT_RS01195 at 276.577 kb on - strand, within BPHYT_RS01195 at 276.577 kb on - strand, within BPHYT_RS01195 at 276.856 kb on - strand, within BPHYT_RS01195 at 277.009 kb on + strand at 277.009 kb on + strand at 277.071 kb on - strand at 277.090 kb on + strand at 277.135 kb on + strand at 277.136 kb on - strand at 277.150 kb on - strand at 277.186 kb on - strand at 277.195 kb on + strand at 277.199 kb on - strand at 277.199 kb on - strand at 277.266 kb on + strand at 277.267 kb on - strand at 277.366 kb on + strand, within BPHYT_RS01200 at 277.408 kb on + strand, within BPHYT_RS01200 at 277.408 kb on + strand, within BPHYT_RS01200 at 277.409 kb on - strand, within BPHYT_RS01200 at 277.594 kb on + strand, within BPHYT_RS01200 at 277.751 kb on - strand, within BPHYT_RS01200 at 277.751 kb on - strand, within BPHYT_RS01200 at 277.754 kb on - strand, within BPHYT_RS01200 at 277.808 kb on - strand, within BPHYT_RS01200 at 277.808 kb on - strand, within BPHYT_RS01200 at 277.892 kb on - strand, within BPHYT_RS01200 at 277.892 kb on - strand, within BPHYT_RS01200 at 278.037 kb on - strand, within BPHYT_RS01200 at 278.080 kb on + strand, within BPHYT_RS01200
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 274,376 - -0.3 274,573 + +0.0 274,659 - +0.0 274,671 - +0.1 274,672 + -0.5 274,702 - -1.7 274,835 + -0.9 274,836 - -0.5 274,887 - +0.5 274,943 + +0.1 275,272 - -0.5 275,276 + -0.0 275,277 - -0.5 275,277 - -1.4 275,304 + -2.8 275,411 + BPHYT_RS01195 0.14 +0.1 275,411 + BPHYT_RS01195 0.14 -0.0 275,412 - BPHYT_RS01195 0.14 -1.7 275,463 + BPHYT_RS01195 0.16 -0.6 275,623 - BPHYT_RS01195 0.25 -2.4 275,886 - BPHYT_RS01195 0.38 -1.0 275,928 + BPHYT_RS01195 0.40 +0.2 276,005 - BPHYT_RS01195 0.44 +0.0 276,005 - BPHYT_RS01195 0.44 -0.7 276,005 - BPHYT_RS01195 0.44 -0.3 276,005 - BPHYT_RS01195 0.44 -0.5 276,008 + BPHYT_RS01195 0.44 +0.2 276,122 + BPHYT_RS01195 0.50 -0.2 276,211 - BPHYT_RS01195 0.54 -0.2 276,440 + BPHYT_RS01195 0.66 -0.1 276,567 + BPHYT_RS01195 0.72 -0.2 276,576 + BPHYT_RS01195 0.73 +1.0 276,577 - BPHYT_RS01195 0.73 -0.4 276,577 - BPHYT_RS01195 0.73 +0.3 276,856 - BPHYT_RS01195 0.87 -0.6 277,009 + +0.7 277,009 + -0.4 277,071 - +0.0 277,090 + +0.4 277,135 + +1.1 277,136 - -0.7 277,150 - -0.8 277,186 - -2.2 277,195 + -0.9 277,199 - +1.3 277,199 - -0.5 277,266 + -0.1 277,267 - +2.2 277,366 + BPHYT_RS01200 0.11 +0.3 277,408 + BPHYT_RS01200 0.13 -0.7 277,408 + BPHYT_RS01200 0.13 +0.1 277,409 - BPHYT_RS01200 0.13 +0.8 277,594 + BPHYT_RS01200 0.23 +0.4 277,751 - BPHYT_RS01200 0.32 -0.3 277,751 - BPHYT_RS01200 0.32 +1.2 277,754 - BPHYT_RS01200 0.32 +0.1 277,808 - BPHYT_RS01200 0.35 +0.9 277,808 - BPHYT_RS01200 0.35 +0.9 277,892 - BPHYT_RS01200 0.39 -0.0 277,892 - BPHYT_RS01200 0.39 +0.3 278,037 - BPHYT_RS01200 0.47 +0.3 278,080 + BPHYT_RS01200 0.50 -0.5
Or see this region's nucleotide sequence