Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00075 and BPHYT_RS00080 overlap by 8 nucleotides BPHYT_RS00080 and BPHYT_RS00085 are separated by 2 nucleotides BPHYT_RS00085 and BPHYT_RS00090 are separated by 204 nucleotides
BPHYT_RS00075: BPHYT_RS00075 - hypothetical protein, at 21,119 to 24,493
_RS00075
BPHYT_RS00080: BPHYT_RS00080 - hypothetical protein, at 24,486 to 25,133
_RS00080
BPHYT_RS00085: BPHYT_RS00085 - hypothetical protein, at 25,136 to 26,590
_RS00085
BPHYT_RS00090: BPHYT_RS00090 - reverse transcriptase, at 26,795 to 28,150
_RS00090
Position (kb) 25
26
27 Strain fitness (log2 ratio) -2
-1
0
1 at 24.138 kb on + strand, within BPHYT_RS00075 at 24.138 kb on + strand, within BPHYT_RS00075 at 24.139 kb on - strand, within BPHYT_RS00075 at 24.266 kb on - strand at 24.267 kb on + strand at 24.267 kb on + strand at 24.267 kb on + strand at 24.267 kb on + strand at 24.267 kb on + strand at 24.268 kb on - strand at 24.409 kb on + strand at 24.485 kb on - strand at 24.533 kb on - strand at 24.779 kb on + strand, within BPHYT_RS00080 at 24.779 kb on + strand, within BPHYT_RS00080 at 24.963 kb on + strand, within BPHYT_RS00080 at 24.988 kb on + strand, within BPHYT_RS00080 at 25.051 kb on + strand, within BPHYT_RS00080 at 25.137 kb on + strand at 25.138 kb on - strand at 25.247 kb on - strand at 25.255 kb on + strand at 25.304 kb on + strand, within BPHYT_RS00085 at 25.360 kb on + strand, within BPHYT_RS00085 at 25.448 kb on + strand, within BPHYT_RS00085 at 25.449 kb on - strand, within BPHYT_RS00085 at 25.488 kb on + strand, within BPHYT_RS00085 at 25.538 kb on - strand, within BPHYT_RS00085 at 25.940 kb on - strand, within BPHYT_RS00085 at 26.122 kb on + strand, within BPHYT_RS00085 at 26.122 kb on + strand, within BPHYT_RS00085 at 26.123 kb on - strand, within BPHYT_RS00085 at 26.123 kb on - strand, within BPHYT_RS00085 at 26.227 kb on + strand, within BPHYT_RS00085 at 26.313 kb on + strand, within BPHYT_RS00085 at 26.358 kb on - strand, within BPHYT_RS00085 at 26.361 kb on + strand, within BPHYT_RS00085 at 26.456 kb on + strand at 26.456 kb on + strand at 26.559 kb on - strand at 26.640 kb on - strand at 26.640 kb on - strand at 26.658 kb on + strand at 26.659 kb on - strand at 26.659 kb on - strand at 26.659 kb on - strand at 27.134 kb on + strand, within BPHYT_RS00090 at 27.547 kb on + strand, within BPHYT_RS00090
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 24,138 + BPHYT_RS00075 0.89 +0.6 24,138 + BPHYT_RS00075 0.89 +0.6 24,139 - BPHYT_RS00075 0.89 +0.1 24,266 - +0.6 24,267 + +0.0 24,267 + +0.0 24,267 + -0.6 24,267 + +0.2 24,267 + +0.1 24,268 - +0.3 24,409 + -0.6 24,485 - +0.2 24,533 - -0.1 24,779 + BPHYT_RS00080 0.45 -1.1 24,779 + BPHYT_RS00080 0.45 -1.0 24,963 + BPHYT_RS00080 0.74 +0.5 24,988 + BPHYT_RS00080 0.77 -1.3 25,051 + BPHYT_RS00080 0.87 -2.1 25,137 + -0.2 25,138 - +1.7 25,247 - +0.6 25,255 + -0.0 25,304 + BPHYT_RS00085 0.12 -0.3 25,360 + BPHYT_RS00085 0.15 -0.1 25,448 + BPHYT_RS00085 0.21 -0.1 25,449 - BPHYT_RS00085 0.22 +0.7 25,488 + BPHYT_RS00085 0.24 -0.1 25,538 - BPHYT_RS00085 0.28 +0.6 25,940 - BPHYT_RS00085 0.55 -2.0 26,122 + BPHYT_RS00085 0.68 -1.7 26,122 + BPHYT_RS00085 0.68 -0.1 26,123 - BPHYT_RS00085 0.68 +0.0 26,123 - BPHYT_RS00085 0.68 +1.0 26,227 + BPHYT_RS00085 0.75 -1.4 26,313 + BPHYT_RS00085 0.81 -0.5 26,358 - BPHYT_RS00085 0.84 -0.6 26,361 + BPHYT_RS00085 0.84 +0.3 26,456 + -2.2 26,456 + -0.0 26,559 - -1.7 26,640 - +1.3 26,640 - +0.1 26,658 + -0.7 26,659 - -1.0 26,659 - +0.3 26,659 - +0.1 27,134 + BPHYT_RS00090 0.25 -0.6 27,547 + BPHYT_RS00090 0.55 +0.2
Or see this region's nucleotide sequence