Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33250 and BPHYT_RS33255 overlap by 4 nucleotides BPHYT_RS33255 and BPHYT_RS33260 are separated by 269 nucleotides BPHYT_RS33260 and BPHYT_RS33265 are separated by 133 nucleotides
BPHYT_RS33250: BPHYT_RS33250 - diguanylate phosphodiesterase, at 3,040,903 to 3,044,016
_RS33250
BPHYT_RS33255: BPHYT_RS33255 - ABC transporter substrate-binding protein, at 3,044,013 to 3,044,807
_RS33255
BPHYT_RS33260: BPHYT_RS33260 - hypothetical protein, at 3,045,077 to 3,045,505
_RS33260
BPHYT_RS33265: BPHYT_RS33265 - Catalase domain-containing protein, at 3,045,639 to 3,046,700
_RS33265
Position (kb)
3044
3045 Strain fitness (log2 ratio)
-2
-1
0
1 at 3043.181 kb on + strand, within BPHYT_RS33250 at 3043.274 kb on + strand, within BPHYT_RS33250 at 3043.310 kb on + strand, within BPHYT_RS33250 at 3043.413 kb on - strand, within BPHYT_RS33250 at 3043.413 kb on - strand, within BPHYT_RS33250 at 3043.424 kb on + strand, within BPHYT_RS33250 at 3043.425 kb on - strand, within BPHYT_RS33250 at 3043.610 kb on + strand, within BPHYT_RS33250 at 3043.611 kb on - strand, within BPHYT_RS33250 at 3043.770 kb on - strand at 3043.892 kb on + strand at 3043.892 kb on + strand at 3044.303 kb on - strand, within BPHYT_RS33255 at 3044.476 kb on + strand, within BPHYT_RS33255 at 3044.479 kb on + strand, within BPHYT_RS33255 at 3044.480 kb on - strand, within BPHYT_RS33255 at 3044.480 kb on - strand, within BPHYT_RS33255 at 3044.494 kb on + strand, within BPHYT_RS33255 at 3044.512 kb on + strand, within BPHYT_RS33255 at 3044.520 kb on - strand, within BPHYT_RS33255 at 3044.520 kb on - strand, within BPHYT_RS33255 at 3044.687 kb on - strand, within BPHYT_RS33255 at 3044.707 kb on - strand, within BPHYT_RS33255 at 3044.942 kb on - strand at 3044.942 kb on - strand at 3044.988 kb on - strand at 3044.995 kb on + strand at 3044.995 kb on + strand at 3045.094 kb on + strand at 3045.111 kb on + strand at 3045.111 kb on + strand at 3045.348 kb on - strand, within BPHYT_RS33260 at 3045.475 kb on + strand at 3045.556 kb on - strand at 3045.731 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 3,043,181 + BPHYT_RS33250 0.73 -0.1 3,043,274 + BPHYT_RS33250 0.76 +0.2 3,043,310 + BPHYT_RS33250 0.77 +0.1 3,043,413 - BPHYT_RS33250 0.81 +0.6 3,043,413 - BPHYT_RS33250 0.81 -0.6 3,043,424 + BPHYT_RS33250 0.81 +0.0 3,043,425 - BPHYT_RS33250 0.81 +1.0 3,043,610 + BPHYT_RS33250 0.87 -0.4 3,043,611 - BPHYT_RS33250 0.87 -0.3 3,043,770 - -0.1 3,043,892 + -0.4 3,043,892 + +0.8 3,044,303 - BPHYT_RS33255 0.36 -1.0 3,044,476 + BPHYT_RS33255 0.58 -0.6 3,044,479 + BPHYT_RS33255 0.59 -1.8 3,044,480 - BPHYT_RS33255 0.59 +1.2 3,044,480 - BPHYT_RS33255 0.59 -2.5 3,044,494 + BPHYT_RS33255 0.61 +1.3 3,044,512 + BPHYT_RS33255 0.63 -1.3 3,044,520 - BPHYT_RS33255 0.64 +1.3 3,044,520 - BPHYT_RS33255 0.64 -0.3 3,044,687 - BPHYT_RS33255 0.85 -0.5 3,044,707 - BPHYT_RS33255 0.87 -1.0 3,044,942 - +0.7 3,044,942 - +0.6 3,044,988 - +0.9 3,044,995 + -0.2 3,044,995 + +0.8 3,045,094 + +0.3 3,045,111 + -0.4 3,045,111 + +0.3 3,045,348 - BPHYT_RS33260 0.63 -0.7 3,045,475 + +1.1 3,045,556 - -1.0 3,045,731 + -0.4
Or see this region's nucleotide sequence