Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31010 and BPHYT_RS31015 overlap by 4 nucleotides BPHYT_RS31015 and BPHYT_RS31020 overlap by 17 nucleotides BPHYT_RS31020 and BPHYT_RS31025 overlap by 4 nucleotides BPHYT_RS31025 and BPHYT_RS31030 are separated by 114 nucleotides
BPHYT_RS31010: BPHYT_RS31010 - arabinose 5-phosphate isomerase, at 2,512,294 to 2,513,229
_RS31010
BPHYT_RS31015: BPHYT_RS31015 - 3-deoxy-manno-octulosonate cytidylyltransferase 2, at 2,513,226 to 2,514,017
_RS31015
BPHYT_RS31020: BPHYT_RS31020 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 2,514,001 to 2,514,555
_RS31020
BPHYT_RS31025: BPHYT_RS31025 - 2-dehydro-3-deoxyphosphooctonate aldolase, at 2,514,552 to 2,515,412
_RS31025
BPHYT_RS31030: BPHYT_RS31030 - tyrosine protein kinase, at 2,515,527 to 2,517,752
_RS31030
Position (kb) 2514
2515 Strain fitness (log2 ratio) -1
0
1 at 2513.214 kb on - strand at 2513.440 kb on + strand, within BPHYT_RS31015 at 2513.441 kb on - strand, within BPHYT_RS31015 at 2514.048 kb on - strand at 2514.237 kb on - strand, within BPHYT_RS31020 at 2515.298 kb on - strand, within BPHYT_RS31025 at 2515.298 kb on - strand, within BPHYT_RS31025
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 2,513,214 - +0.9 2,513,440 + BPHYT_RS31015 0.27 -1.0 2,513,441 - BPHYT_RS31015 0.27 -1.6 2,514,048 - -0.3 2,514,237 - BPHYT_RS31020 0.43 +0.1 2,515,298 - BPHYT_RS31025 0.87 +0.7 2,515,298 - BPHYT_RS31025 0.87 +0.4
Or see this region's nucleotide sequence