Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27575

Experiment: 36hr PHYTO8 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27570 and BPHYT_RS27575 are separated by 192 nucleotidesBPHYT_RS27575 and BPHYT_RS27580 overlap by 4 nucleotides BPHYT_RS27570: BPHYT_RS27570 - universal stress protein UspA, at 1,758,543 to 1,759,040 _RS27570 BPHYT_RS27575: BPHYT_RS27575 - ATP synthase F1 subunit gamma, at 1,759,233 to 1,760,081 _RS27575 BPHYT_RS27580: BPHYT_RS27580 - F0F1 ATP synthase subunit alpha, at 1,760,078 to 1,761,646 _RS27580 Position (kb) 1759 1760 1761Strain fitness (log2 ratio) -1 0 1 2at 1758.310 kb on + strandat 1758.311 kb on - strandat 1758.627 kb on - strand, within BPHYT_RS27570at 1758.890 kb on - strand, within BPHYT_RS27570at 1758.929 kb on + strand, within BPHYT_RS27570at 1759.037 kb on - strandat 1759.429 kb on + strand, within BPHYT_RS27575at 1759.429 kb on + strand, within BPHYT_RS27575at 1759.435 kb on + strand, within BPHYT_RS27575at 1759.560 kb on - strand, within BPHYT_RS27575at 1759.788 kb on + strand, within BPHYT_RS27575at 1760.481 kb on + strand, within BPHYT_RS27580at 1760.895 kb on + strand, within BPHYT_RS27580at 1760.995 kb on - strand, within BPHYT_RS27580

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10
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1,758,310 + +2.2
1,758,311 - +0.1
1,758,627 - BPHYT_RS27570 0.17 +1.6
1,758,890 - BPHYT_RS27570 0.70 +0.4
1,758,929 + BPHYT_RS27570 0.78 -1.3
1,759,037 - +0.4
1,759,429 + BPHYT_RS27575 0.23 -0.3
1,759,429 + BPHYT_RS27575 0.23 +1.3
1,759,435 + BPHYT_RS27575 0.24 +0.0
1,759,560 - BPHYT_RS27575 0.39 -1.3
1,759,788 + BPHYT_RS27575 0.65 +1.0
1,760,481 + BPHYT_RS27580 0.26 +0.2
1,760,895 + BPHYT_RS27580 0.52 +1.4
1,760,995 - BPHYT_RS27580 0.58 -0.8

Or see this region's nucleotide sequence