Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21800 and BPHYT_RS21805 are separated by 200 nucleotides BPHYT_RS21805 and BPHYT_RS21810 overlap by 4 nucleotides BPHYT_RS21810 and BPHYT_RS21815 are separated by 69 nucleotides BPHYT_RS21815 and BPHYT_RS21820 are separated by 290 nucleotides
BPHYT_RS21800: BPHYT_RS21800 - hypothetical protein, at 447,590 to 447,892
_RS21800
BPHYT_RS21805: BPHYT_RS21805 - transcriptional regulator, at 448,093 to 448,773
_RS21805
BPHYT_RS21810: BPHYT_RS21810 - histidine kinase, at 448,770 to 450,215
_RS21810
BPHYT_RS21815: BPHYT_RS21815 - hypothetical protein, at 450,285 to 450,557
_RS21815
BPHYT_RS21820: BPHYT_RS21820 - hypothetical protein, at 450,848 to 451,132
_RS21820
Position (kb) 448
449
450
451 Strain fitness (log2 ratio) -2
-1
0
1 at 448.247 kb on + strand, within BPHYT_RS21805 at 448.248 kb on - strand, within BPHYT_RS21805 at 448.248 kb on - strand, within BPHYT_RS21805 at 448.832 kb on + strand at 448.869 kb on + strand at 448.936 kb on + strand, within BPHYT_RS21810 at 449.260 kb on - strand, within BPHYT_RS21810 at 449.319 kb on + strand, within BPHYT_RS21810 at 449.320 kb on - strand, within BPHYT_RS21810 at 449.989 kb on + strand, within BPHYT_RS21810 at 449.989 kb on + strand, within BPHYT_RS21810 at 449.990 kb on - strand, within BPHYT_RS21810 at 450.358 kb on - strand, within BPHYT_RS21815 at 450.860 kb on - strand at 451.143 kb on - strand at 451.192 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 448,247 + BPHYT_RS21805 0.23 -1.9 448,248 - BPHYT_RS21805 0.23 -2.7 448,248 - BPHYT_RS21805 0.23 -0.2 448,832 + +0.9 448,869 + -0.9 448,936 + BPHYT_RS21810 0.11 +0.0 449,260 - BPHYT_RS21810 0.34 -1.2 449,319 + BPHYT_RS21810 0.38 +1.1 449,320 - BPHYT_RS21810 0.38 +1.7 449,989 + BPHYT_RS21810 0.84 -0.1 449,989 + BPHYT_RS21810 0.84 -0.6 449,990 - BPHYT_RS21810 0.84 -1.2 450,358 - BPHYT_RS21815 0.27 +1.2 450,860 - -0.7 451,143 - +0.2 451,192 + +0.2
Or see this region's nucleotide sequence