Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21315 and BPHYT_RS21320 are separated by 216 nucleotides BPHYT_RS21320 and BPHYT_RS21325 are separated by 229 nucleotides BPHYT_RS21325 and BPHYT_RS21330 are separated by 384 nucleotides
BPHYT_RS21315: BPHYT_RS21315 - hypothetical protein, at 341,401 to 341,937
_RS21315
BPHYT_RS21320: BPHYT_RS21320 - alkaline phosphatase, at 342,154 to 344,106
_RS21320
BPHYT_RS21325: BPHYT_RS21325 - hypothetical protein, at 344,336 to 344,674
_RS21325
BPHYT_RS21330: BPHYT_RS21330 - hypothetical protein, at 345,059 to 345,493
_RS21330
Position (kb) 342
343
344
345 Strain fitness (log2 ratio) -2
-1
0
1
2 at 341.245 kb on + strand at 341.245 kb on + strand at 341.245 kb on + strand at 341.727 kb on + strand, within BPHYT_RS21315 at 341.809 kb on + strand, within BPHYT_RS21315 at 341.833 kb on - strand, within BPHYT_RS21315 at 341.833 kb on - strand, within BPHYT_RS21315 at 341.872 kb on - strand, within BPHYT_RS21315 at 341.872 kb on - strand, within BPHYT_RS21315 at 342.010 kb on + strand at 342.010 kb on + strand at 342.014 kb on - strand at 342.014 kb on - strand at 342.026 kb on + strand at 342.028 kb on + strand at 342.029 kb on - strand at 342.048 kb on - strand at 342.048 kb on - strand at 342.439 kb on + strand, within BPHYT_RS21320 at 342.440 kb on - strand, within BPHYT_RS21320 at 342.571 kb on + strand, within BPHYT_RS21320 at 342.703 kb on + strand, within BPHYT_RS21320 at 342.703 kb on + strand, within BPHYT_RS21320 at 342.704 kb on - strand, within BPHYT_RS21320 at 342.781 kb on + strand, within BPHYT_RS21320 at 342.796 kb on - strand, within BPHYT_RS21320 at 343.031 kb on - strand, within BPHYT_RS21320 at 343.171 kb on + strand, within BPHYT_RS21320 at 343.171 kb on + strand, within BPHYT_RS21320 at 343.172 kb on - strand, within BPHYT_RS21320 at 343.336 kb on + strand, within BPHYT_RS21320 at 343.351 kb on + strand, within BPHYT_RS21320 at 343.351 kb on + strand, within BPHYT_RS21320 at 343.352 kb on - strand, within BPHYT_RS21320 at 343.352 kb on - strand, within BPHYT_RS21320 at 343.426 kb on + strand, within BPHYT_RS21320 at 343.485 kb on + strand, within BPHYT_RS21320 at 343.485 kb on + strand, within BPHYT_RS21320 at 343.502 kb on + strand, within BPHYT_RS21320 at 343.502 kb on + strand, within BPHYT_RS21320 at 343.502 kb on + strand, within BPHYT_RS21320 at 343.502 kb on + strand, within BPHYT_RS21320 at 343.503 kb on - strand, within BPHYT_RS21320 at 343.503 kb on - strand, within BPHYT_RS21320 at 343.503 kb on - strand, within BPHYT_RS21320 at 343.601 kb on - strand, within BPHYT_RS21320 at 343.601 kb on - strand, within BPHYT_RS21320 at 343.849 kb on + strand, within BPHYT_RS21320 at 343.948 kb on + strand at 344.042 kb on - strand at 344.042 kb on - strand at 344.042 kb on - strand at 344.042 kb on - strand at 344.104 kb on + strand at 344.104 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.235 kb on - strand at 344.237 kb on - strand at 344.237 kb on - strand at 344.259 kb on - strand at 344.314 kb on + strand at 344.353 kb on + strand at 344.474 kb on + strand, within BPHYT_RS21325 at 344.531 kb on + strand, within BPHYT_RS21325 at 344.531 kb on + strand, within BPHYT_RS21325 at 344.663 kb on + strand at 344.748 kb on + strand at 344.812 kb on + strand at 344.812 kb on + strand at 344.868 kb on - strand at 344.943 kb on + strand at 344.943 kb on + strand at 344.944 kb on - strand at 344.984 kb on + strand at 344.984 kb on + strand at 344.984 kb on + strand at 344.985 kb on - strand at 344.987 kb on - strand at 344.994 kb on - strand at 345.009 kb on + strand at 345.009 kb on + strand at 345.009 kb on + strand at 345.009 kb on + strand at 345.010 kb on - strand at 345.010 kb on - strand at 345.086 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 341,245 + +0.4 341,245 + -0.2 341,245 + -0.3 341,727 + BPHYT_RS21315 0.61 -1.0 341,809 + BPHYT_RS21315 0.76 +0.5 341,833 - BPHYT_RS21315 0.80 -0.7 341,833 - BPHYT_RS21315 0.80 -1.1 341,872 - BPHYT_RS21315 0.88 -1.2 341,872 - BPHYT_RS21315 0.88 -0.3 342,010 + -0.5 342,010 + +0.2 342,014 - -0.4 342,014 - +0.3 342,026 + +0.0 342,028 + +1.9 342,029 - -0.6 342,048 - -1.3 342,048 - +1.1 342,439 + BPHYT_RS21320 0.15 +0.7 342,440 - BPHYT_RS21320 0.15 +0.1 342,571 + BPHYT_RS21320 0.21 -1.7 342,703 + BPHYT_RS21320 0.28 -1.2 342,703 + BPHYT_RS21320 0.28 -0.6 342,704 - BPHYT_RS21320 0.28 -0.3 342,781 + BPHYT_RS21320 0.32 +0.6 342,796 - BPHYT_RS21320 0.33 +0.1 343,031 - BPHYT_RS21320 0.45 +0.3 343,171 + BPHYT_RS21320 0.52 +0.1 343,171 + BPHYT_RS21320 0.52 +2.1 343,172 - BPHYT_RS21320 0.52 -0.1 343,336 + BPHYT_RS21320 0.61 -0.2 343,351 + BPHYT_RS21320 0.61 +0.4 343,351 + BPHYT_RS21320 0.61 -0.9 343,352 - BPHYT_RS21320 0.61 -1.3 343,352 - BPHYT_RS21320 0.61 +1.7 343,426 + BPHYT_RS21320 0.65 +0.4 343,485 + BPHYT_RS21320 0.68 -0.9 343,485 + BPHYT_RS21320 0.68 -0.3 343,502 + BPHYT_RS21320 0.69 +0.2 343,502 + BPHYT_RS21320 0.69 -0.2 343,502 + BPHYT_RS21320 0.69 +0.9 343,502 + BPHYT_RS21320 0.69 -0.7 343,503 - BPHYT_RS21320 0.69 -1.0 343,503 - BPHYT_RS21320 0.69 -1.0 343,503 - BPHYT_RS21320 0.69 +0.4 343,601 - BPHYT_RS21320 0.74 -0.5 343,601 - BPHYT_RS21320 0.74 +1.1 343,849 + BPHYT_RS21320 0.87 -0.8 343,948 + -0.9 344,042 - -0.3 344,042 - +0.0 344,042 - -1.2 344,042 - +1.0 344,104 + -0.5 344,104 + +0.6 344,234 + -1.1 344,234 + +0.8 344,234 + -0.5 344,234 + -1.1 344,234 + +2.7 344,234 + +0.7 344,235 - -1.7 344,237 - +0.1 344,237 - +0.4 344,259 - -2.2 344,314 + +0.1 344,353 + -1.2 344,474 + BPHYT_RS21325 0.41 +0.2 344,531 + BPHYT_RS21325 0.58 -0.3 344,531 + BPHYT_RS21325 0.58 -0.9 344,663 + -0.0 344,748 + +0.6 344,812 + +0.6 344,812 + +0.9 344,868 - -1.0 344,943 + -0.0 344,943 + +1.1 344,944 - +0.3 344,984 + +0.7 344,984 + +0.2 344,984 + +0.2 344,985 - -0.7 344,987 - -1.6 344,994 - -1.4 345,009 + +0.4 345,009 + -0.5 345,009 + +0.4 345,009 + -0.4 345,010 - +0.7 345,010 - +1.4 345,086 - -0.1
Or see this region's nucleotide sequence