Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20805

Experiment: 36hr PHYTO8 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20800 and BPHYT_RS20805 are separated by 126 nucleotidesBPHYT_RS20805 and BPHYT_RS20810 are separated by 59 nucleotides BPHYT_RS20800: BPHYT_RS20800 - alanine-phosphoribitol ligase, at 228,645 to 230,318 _RS20800 BPHYT_RS20805: BPHYT_RS20805 - LacI family transcriptional regulator, at 230,445 to 231,524 _RS20805 BPHYT_RS20810: BPHYT_RS20810 - ABC transporter permease, at 231,584 to 232,549 _RS20810 Position (kb) 230 231 232Strain fitness (log2 ratio) -2 -1 0 1at 229.565 kb on + strand, within BPHYT_RS20800at 229.565 kb on + strand, within BPHYT_RS20800at 229.565 kb on + strand, within BPHYT_RS20800at 229.791 kb on + strand, within BPHYT_RS20800at 230.020 kb on - strand, within BPHYT_RS20800at 230.369 kb on - strandat 230.817 kb on + strand, within BPHYT_RS20805at 230.817 kb on + strand, within BPHYT_RS20805at 230.818 kb on - strand, within BPHYT_RS20805at 232.142 kb on - strand, within BPHYT_RS20810at 232.289 kb on + strand, within BPHYT_RS20810at 232.290 kb on - strand, within BPHYT_RS20810

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10
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229,565 + BPHYT_RS20800 0.55 +1.1
229,565 + BPHYT_RS20800 0.55 +0.3
229,565 + BPHYT_RS20800 0.55 -0.6
229,791 + BPHYT_RS20800 0.68 +0.2
230,020 - BPHYT_RS20800 0.82 +0.5
230,369 - -0.2
230,817 + BPHYT_RS20805 0.34 -0.1
230,817 + BPHYT_RS20805 0.34 -2.4
230,818 - BPHYT_RS20805 0.35 -0.5
232,142 - BPHYT_RS20810 0.58 +0.2
232,289 + BPHYT_RS20810 0.73 -0.8
232,290 - BPHYT_RS20810 0.73 -1.8

Or see this region's nucleotide sequence