Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20790 and BPHYT_RS20795 are separated by 246 nucleotides BPHYT_RS20795 and BPHYT_RS20800 are separated by 95 nucleotides
BPHYT_RS20790: BPHYT_RS20790 - TetR family transcriptional regulator, at 226,144 to 226,794
_RS20790
BPHYT_RS20795: BPHYT_RS20795 - sorbosone dehydrogenase, at 227,041 to 228,549
_RS20795
BPHYT_RS20800: BPHYT_RS20800 - alanine-phosphoribitol ligase, at 228,645 to 230,318
_RS20800
Position (kb) 227
228
229 Strain fitness (log2 ratio) -2
-1
0
1
2 at 226.571 kb on + strand, within BPHYT_RS20790 at 226.625 kb on + strand, within BPHYT_RS20790 at 226.897 kb on - strand at 227.425 kb on + strand, within BPHYT_RS20795 at 227.634 kb on + strand, within BPHYT_RS20795 at 227.635 kb on - strand, within BPHYT_RS20795 at 227.742 kb on + strand, within BPHYT_RS20795 at 227.742 kb on + strand, within BPHYT_RS20795 at 227.743 kb on - strand, within BPHYT_RS20795 at 228.270 kb on + strand, within BPHYT_RS20795 at 228.491 kb on - strand at 228.491 kb on - strand at 228.622 kb on - strand at 228.648 kb on + strand at 228.648 kb on + strand at 228.648 kb on + strand at 228.648 kb on + strand at 228.654 kb on + strand at 228.654 kb on + strand at 228.765 kb on + strand at 228.767 kb on + strand at 228.943 kb on - strand, within BPHYT_RS20800 at 229.143 kb on + strand, within BPHYT_RS20800 at 229.143 kb on + strand, within BPHYT_RS20800
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 226,571 + BPHYT_RS20790 0.66 -0.3 226,625 + BPHYT_RS20790 0.74 -0.3 226,897 - +0.7 227,425 + BPHYT_RS20795 0.25 +0.5 227,634 + BPHYT_RS20795 0.39 +1.7 227,635 - BPHYT_RS20795 0.39 -0.1 227,742 + BPHYT_RS20795 0.46 -0.9 227,742 + BPHYT_RS20795 0.46 +0.2 227,743 - BPHYT_RS20795 0.47 -0.6 228,270 + BPHYT_RS20795 0.81 -0.5 228,491 - -0.4 228,491 - +2.4 228,622 - -0.8 228,648 + -0.5 228,648 + -2.1 228,648 + +0.2 228,648 + +0.3 228,654 + +1.7 228,654 + +1.7 228,765 + +0.6 228,767 + +0.1 228,943 - BPHYT_RS20800 0.18 -0.1 229,143 + BPHYT_RS20800 0.30 -1.8 229,143 + BPHYT_RS20800 0.30 -1.3
Or see this region's nucleotide sequence