Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS18335

Experiment: 36hr PHYTO8 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS18330 and BPHYT_RS18335 are separated by 216 nucleotidesBPHYT_RS18335 and BPHYT_RS18340 are separated by 184 nucleotides BPHYT_RS18330: BPHYT_RS18330 - phosphatidylethanolamine-binding protein, at 4,123,985 to 4,124,587 _RS18330 BPHYT_RS18335: BPHYT_RS18335 - NADPH-dependent FMN reductase, at 4,124,804 to 4,125,376 _RS18335 BPHYT_RS18340: BPHYT_RS18340 - n-acetyl-gamma-glutamyl-phosphate reductase, at 4,125,561 to 4,126,508 _RS18340 Position (kb) 4124 4125 4126Strain fitness (log2 ratio) -2 -1 0 1 2at 4123.834 kb on + strandat 4123.893 kb on - strandat 4123.893 kb on - strandat 4123.893 kb on - strandat 4123.893 kb on - strandat 4123.893 kb on - strandat 4123.893 kb on - strandat 4123.899 kb on - strandat 4123.937 kb on - strandat 4124.166 kb on + strand, within BPHYT_RS18330at 4124.166 kb on + strand, within BPHYT_RS18330at 4124.166 kb on + strand, within BPHYT_RS18330at 4124.365 kb on + strand, within BPHYT_RS18330at 4124.453 kb on + strand, within BPHYT_RS18330at 4124.453 kb on + strand, within BPHYT_RS18330at 4124.468 kb on + strand, within BPHYT_RS18330at 4124.577 kb on - strandat 4124.585 kb on + strandat 4124.586 kb on - strandat 4124.586 kb on - strandat 4124.657 kb on + strandat 4124.658 kb on - strandat 4124.696 kb on + strandat 4124.697 kb on - strandat 4124.826 kb on - strandat 4124.933 kb on - strand, within BPHYT_RS18335at 4124.987 kb on + strand, within BPHYT_RS18335at 4124.988 kb on - strand, within BPHYT_RS18335at 4124.988 kb on - strand, within BPHYT_RS18335at 4124.988 kb on - strand, within BPHYT_RS18335at 4125.227 kb on + strand, within BPHYT_RS18335at 4125.228 kb on - strand, within BPHYT_RS18335at 4125.228 kb on - strand, within BPHYT_RS18335at 4125.228 kb on - strand, within BPHYT_RS18335at 4125.228 kb on - strand, within BPHYT_RS18335at 4125.228 kb on - strand, within BPHYT_RS18335at 4125.228 kb on - strand, within BPHYT_RS18335at 4125.228 kb on - strand, within BPHYT_RS18335at 4125.228 kb on - strand, within BPHYT_RS18335at 4125.336 kb on + strandat 4126.098 kb on - strand, within BPHYT_RS18340at 4126.266 kb on + strand, within BPHYT_RS18340

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10
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4,123,834 + +1.9
4,123,893 - -0.5
4,123,893 - +1.3
4,123,893 - +0.4
4,123,893 - -1.2
4,123,893 - +0.6
4,123,893 - -0.1
4,123,899 - -2.2
4,123,937 - +0.9
4,124,166 + BPHYT_RS18330 0.30 +0.1
4,124,166 + BPHYT_RS18330 0.30 -0.7
4,124,166 + BPHYT_RS18330 0.30 -0.3
4,124,365 + BPHYT_RS18330 0.63 -0.7
4,124,453 + BPHYT_RS18330 0.78 -0.2
4,124,453 + BPHYT_RS18330 0.78 -1.4
4,124,468 + BPHYT_RS18330 0.80 -0.4
4,124,577 - +0.0
4,124,585 + -0.8
4,124,586 - +0.3
4,124,586 - -0.5
4,124,657 + +0.5
4,124,658 - +0.1
4,124,696 + -0.3
4,124,697 - +0.8
4,124,826 - -0.2
4,124,933 - BPHYT_RS18335 0.23 +0.3
4,124,987 + BPHYT_RS18335 0.32 +0.4
4,124,988 - BPHYT_RS18335 0.32 -0.7
4,124,988 - BPHYT_RS18335 0.32 -0.5
4,124,988 - BPHYT_RS18335 0.32 +0.0
4,125,227 + BPHYT_RS18335 0.74 -0.2
4,125,228 - BPHYT_RS18335 0.74 -0.0
4,125,228 - BPHYT_RS18335 0.74 -0.1
4,125,228 - BPHYT_RS18335 0.74 +0.5
4,125,228 - BPHYT_RS18335 0.74 -0.1
4,125,228 - BPHYT_RS18335 0.74 +2.6
4,125,228 - BPHYT_RS18335 0.74 -0.2
4,125,228 - BPHYT_RS18335 0.74 +0.2
4,125,228 - BPHYT_RS18335 0.74 +0.1
4,125,336 + -1.1
4,126,098 - BPHYT_RS18340 0.57 +0.0
4,126,266 + BPHYT_RS18340 0.74 -1.0

Or see this region's nucleotide sequence