Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15835 and BPHYT_RS15840 are separated by 295 nucleotides BPHYT_RS15840 and BPHYT_RS15845 are separated by 243 nucleotides
BPHYT_RS15835: BPHYT_RS15835 - RNA polymerase subunit sigma24, at 3,595,002 to 3,595,988
_RS15835
BPHYT_RS15840: BPHYT_RS15840 - Clp protease proteolytic subunit ClpP, at 3,596,284 to 3,596,931
_RS15840
BPHYT_RS15845: BPHYT_RS15845 - hypothetical protein, at 3,597,175 to 3,598,611
_RS15845
Position (kb)
3596
3597 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3595.660 kb on + strand, within BPHYT_RS15835 at 3595.660 kb on + strand, within BPHYT_RS15835 at 3595.828 kb on - strand, within BPHYT_RS15835 at 3595.988 kb on + strand at 3595.988 kb on + strand at 3595.989 kb on - strand at 3595.992 kb on + strand at 3595.993 kb on - strand at 3595.993 kb on - strand at 3595.993 kb on - strand at 3596.284 kb on - strand at 3596.284 kb on - strand at 3596.284 kb on - strand at 3596.296 kb on + strand at 3596.308 kb on - strand at 3596.375 kb on - strand, within BPHYT_RS15840 at 3596.490 kb on + strand, within BPHYT_RS15840 at 3596.490 kb on + strand, within BPHYT_RS15840 at 3596.491 kb on - strand, within BPHYT_RS15840 at 3596.527 kb on + strand, within BPHYT_RS15840 at 3596.527 kb on + strand, within BPHYT_RS15840 at 3596.546 kb on - strand, within BPHYT_RS15840 at 3596.747 kb on - strand, within BPHYT_RS15840 at 3596.860 kb on + strand, within BPHYT_RS15840 at 3596.914 kb on + strand at 3596.925 kb on + strand at 3596.926 kb on - strand at 3596.926 kb on - strand at 3596.930 kb on - strand at 3596.961 kb on + strand at 3597.210 kb on - strand at 3597.270 kb on + strand at 3597.329 kb on - strand, within BPHYT_RS15845 at 3597.346 kb on + strand, within BPHYT_RS15845 at 3597.632 kb on - strand, within BPHYT_RS15845 at 3597.748 kb on - strand, within BPHYT_RS15845 at 3597.901 kb on + strand, within BPHYT_RS15845
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 3,595,660 + BPHYT_RS15835 0.67 -0.8 3,595,660 + BPHYT_RS15835 0.67 -1.7 3,595,828 - BPHYT_RS15835 0.84 +0.0 3,595,988 + -0.6 3,595,988 + +0.6 3,595,989 - -1.4 3,595,992 + +0.6 3,595,993 - +0.5 3,595,993 - +1.1 3,595,993 - +3.5 3,596,284 - -1.0 3,596,284 - -0.5 3,596,284 - +0.5 3,596,296 + +0.1 3,596,308 - -0.8 3,596,375 - BPHYT_RS15840 0.14 -0.1 3,596,490 + BPHYT_RS15840 0.32 +0.0 3,596,490 + BPHYT_RS15840 0.32 +2.3 3,596,491 - BPHYT_RS15840 0.32 -0.3 3,596,527 + BPHYT_RS15840 0.38 +0.4 3,596,527 + BPHYT_RS15840 0.38 -1.8 3,596,546 - BPHYT_RS15840 0.40 -0.6 3,596,747 - BPHYT_RS15840 0.71 -1.3 3,596,860 + BPHYT_RS15840 0.89 -1.2 3,596,914 + -1.0 3,596,925 + +0.7 3,596,926 - -0.8 3,596,926 - +0.1 3,596,930 - -0.4 3,596,961 + -0.7 3,597,210 - -0.4 3,597,270 + -1.0 3,597,329 - BPHYT_RS15845 0.11 +1.8 3,597,346 + BPHYT_RS15845 0.12 -0.0 3,597,632 - BPHYT_RS15845 0.32 -0.0 3,597,748 - BPHYT_RS15845 0.40 +0.6 3,597,901 + BPHYT_RS15845 0.51 +0.6
Or see this region's nucleotide sequence