Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10705

Experiment: 36hr PHYTO8 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10690 and BPHYT_RS10695 overlap by 4 nucleotidesBPHYT_RS10695 and BPHYT_RS10700 are separated by 32 nucleotidesBPHYT_RS10700 and BPHYT_RS10705 are separated by 40 nucleotidesBPHYT_RS10705 and BPHYT_RS10710 are separated by 10 nucleotides BPHYT_RS10690: BPHYT_RS10690 - Rieske (2Fe-2S) protein, at 2,433,692 to 2,434,975 _RS10690 BPHYT_RS10695: BPHYT_RS10695 - aromatic-ring-hydroxylating dioxygenase subunit beta, at 2,434,972 to 2,435,472 _RS10695 BPHYT_RS10700: BPHYT_RS10700 - 4-oxalocrotonate tautomerase, at 2,435,505 to 2,435,888 _RS10700 BPHYT_RS10705: BPHYT_RS10705 - MFS transporter, at 2,435,929 to 2,437,245 _RS10705 BPHYT_RS10710: BPHYT_RS10710 - sensor histidine kinase, at 2,437,256 to 2,438,776 _RS10710 Position (kb) 2435 2436 2437 2438Strain fitness (log2 ratio) -1 0 1 2at 2435.108 kb on - strand, within BPHYT_RS10695at 2435.510 kb on + strandat 2435.511 kb on - strandat 2436.430 kb on + strand, within BPHYT_RS10705at 2436.877 kb on + strand, within BPHYT_RS10705at 2436.878 kb on - strand, within BPHYT_RS10705at 2436.879 kb on + strand, within BPHYT_RS10705at 2437.132 kb on + strandat 2437.132 kb on + strandat 2437.227 kb on + strandat 2437.645 kb on - strand, within BPHYT_RS10710at 2437.980 kb on + strand, within BPHYT_RS10710

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10
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2,435,108 - BPHYT_RS10695 0.27 -0.9
2,435,510 + -0.3
2,435,511 - +1.2
2,436,430 + BPHYT_RS10705 0.38 -0.4
2,436,877 + BPHYT_RS10705 0.72 +0.9
2,436,878 - BPHYT_RS10705 0.72 +0.6
2,436,879 + BPHYT_RS10705 0.72 +2.6
2,437,132 + +0.6
2,437,132 + -1.7
2,437,227 + -0.7
2,437,645 - BPHYT_RS10710 0.26 -1.3
2,437,980 + BPHYT_RS10710 0.48 -1.0

Or see this region's nucleotide sequence